Oxaliplatin but Not Irinotecan Impairs Posthepatectomy Liver Regeneration in a Murine Model

Abstract EN

Introduction.

We examined the murine hepatectomy model of liver regeneration (LR) in the setting of neoadjuvant chemotherapy.

Methods.

C57BL/6 mice were randomized to receive neoadjuvant intraperitoneal (IP) injections of a control, oxaliplatin (15 mg/kg), or irinotecan (100 mg/Kg or 250 mg/Kg) solution.

Hepatectomy (70%) was performed 14 days after the final IP treatment.

Animals were sacrificed at postoperative day (D) 0, 1, 2, 3, and 7.

Liver remnants and serum were collected for analysis.

T-tests for independent samples were used for statistical comparisons.

Results.

For oxaliplatin, percent LR did not differ at D1 or D2 but was significantly less at D3 (89.0% versus 70.0%, P=0.048) with no difference on D7 (P=0.21).

Irinotecan-treated mice at both dose levels (100 mg/Kg and 250 mg/Kg) showed no significant differences in LR.

BrdU incorporation was significantly decreased in oxaliplatin-treated animals (D1,2,3).

Conclusions.

Neoadjuvant oxaliplatin but not irinotecan impairs early LR in a posthepatectomy murine model which correlates with decreased DNA synthesis.