An MFS Transporter-Like ORF from MDR Acinetobacter baumannii AIIMS 7 Is Associated with Adherence and Biofilm Formation on BioticAbiotic Surface
Joint Authors
Iyer, Pavithra S.
Sahu, Praveen K.
Talreja, Sheetal C.
Pardesi, Karishma R.
Chopade, Balu A.
Gaikwad, Madhumita B.
Source
International Journal of Microbiology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
A major facilitator superfamily (MFS) transporter-like open reading frame (ORF) of 453 bp was identified in a pathogenic strain Acinetobacter baumannii AIIMS 7, and its association with adherence and biofilm formation was investigated.
Reverse transcription PCR (RT-PCR) showed differential expression in surface-attached biofilm cells than nonadherent cells.
In vitro translation showed synthesis of a ~17 kDa protein, further confirmed by cloning and heterologous expression in E.
coli DH5α.
Up to 2.1-, 3.1-, and 4.1- fold biofilm augmentation was observed on abiotic (polystyrene) and biotic (S.
cerevisiae/HeLa) surface, respectively.
Scanning electron microscopy (SEM) and gfp-tagged fluorescence microscopy revealed increased adherence to abiotic (glass) and biotic (S.
cerevisiae) surface.
Extracellular DNA(eDNA) was found significantly during active growth; due to probable involvement of the protein in DNA export, strong sequence homology with MFS transporter proteins, and presence of transmembrane helices.
In summary, our findings show that the putative MFS transporter-like ORF (pmt) is associated with adherence, biofilm formation, and probable eDNA release in A.
baumannii AIIMS 7.
American Psychological Association (APA)
Sahu, Praveen K.& Iyer, Pavithra S.& Gaikwad, Madhumita B.& Talreja, Sheetal C.& Pardesi, Karishma R.& Chopade, Balu A.. 2012. An MFS Transporter-Like ORF from MDR Acinetobacter baumannii AIIMS 7 Is Associated with Adherence and Biofilm Formation on BioticAbiotic Surface. International Journal of Microbiology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-475789
Modern Language Association (MLA)
Sahu, Praveen K.…[et al.]. An MFS Transporter-Like ORF from MDR Acinetobacter baumannii AIIMS 7 Is Associated with Adherence and Biofilm Formation on BioticAbiotic Surface. International Journal of Microbiology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-475789
American Medical Association (AMA)
Sahu, Praveen K.& Iyer, Pavithra S.& Gaikwad, Madhumita B.& Talreja, Sheetal C.& Pardesi, Karishma R.& Chopade, Balu A.. An MFS Transporter-Like ORF from MDR Acinetobacter baumannii AIIMS 7 Is Associated with Adherence and Biofilm Formation on BioticAbiotic Surface. International Journal of Microbiology. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-475789
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475789