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Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA
Joint Authors
Malcolm, C.
Williams, C. L.
MacKay, W. G.
McMillan, M.
Weaver, L. T.
Shepherd, Ashley
Source
Gastroenterology Research and Practice
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Helicobacter pylori infection, often acquired in early childhood, is a global cause of undernutrition, gastritis, peptic ulcer disease and gastric carcinoma.
This study tested the feasibility of using H.
pylori shed in the faeces as a source of DNA for non-invasive epidemiological studies.
H.
pylori DNA was chemically recovered and isolated using a specific biotinylated oligonucleotide probe with magnetic capture from 28 H.
pylori positive faecal samples obtained from children attending hospital for the investigation of suspected H.
pylori infection, together with close family members.
Random amplification of polymorphic DNA (RAPD) was subsequently used to discriminate each isolate.
93% of stool samples selected were typeable.
Parent, child and sibling samples were compared and similarities determined.
Phylogenetic analysis showed that H.
pylori DNA obtained from the faeces can be used to genotype individual strains, offering a means of studying intrafamilial transfer of this microorganism.
American Psychological Association (APA)
McMillan, M.& MacKay, W. G.& Williams, C. L.& Shepherd, Ashley& Malcolm, C.& Weaver, L. T.. 2011. Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA. Gastroenterology Research and Practice،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-475827
Modern Language Association (MLA)
McMillan, M.…[et al.]. Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA. Gastroenterology Research and Practice No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-475827
American Medical Association (AMA)
McMillan, M.& MacKay, W. G.& Williams, C. L.& Shepherd, Ashley& Malcolm, C.& Weaver, L. T.. Intrafamilial Genotyping of Helicobacter pylori from Faecal DNA. Gastroenterology Research and Practice. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-475827
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475827