Antiproliferative Activity of Cinnamomum cassia Constituents and Effects of Pifithrin-Alpha on Their Apoptotic Signaling Pathways in Hep G2 Cells
Joint Authors
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-02
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Cinnamaldehyde (Cin), cinnamic acid (Ca) and cinnamyl alcohol (Cal), major constituents of Cinnamomum cassia, have been shown to possess antioxidant, anti-inflammatory, anticancer and other activities.
In this study, our aim was to evaluate the antiproliferative activity of these compounds in human hepatoma Hep G2 cells and examine the effects of pifithrin-alpha (PFTα; a specific p53 inhibitor) on their apoptotic signaling transduction mechanism.
The antiproliferative activity was measured by XTT assay.
Expression of apoptosis-related proteins was detected by western blotting.
Results showed that at a concentration of 30 μM, the order of antiproliferative activity in Hep G2 cells was Cin > Ca > Cal.
Cin (IC50 9.76 ± 0.67 μM) demonstrated an antiproliferative potency as good as 5-fluorouracil (an anti-cancer drug; IC50 9.57 ± 0.61 μM).
Further studies on apoptotic mechanisms of Cin showed that it downregulated the expression of Bcl-XL, upregulated CD95 (APO-1), p53 and Bax proteins, as well as cleaving the poly (ADP-ribose) polymerase (PARP) in a time-dependent pattern.
PFTα pre-incubation significantly diminished the effect of Cin-induced apoptosis.
It markedly upregulated the anti-apoptotic (Bcl-XL) expression and downregulated the pro-apoptotic (Bax) expression, as well as effectively blocking the CD95 (APO-1) and p53 expression, and PARP cleavage in Cin-treated cells.
This study indicates that Cin was the most potent antiproliferative constituent of C.
cassia, and its apoptotic mechanism in Hep G2 cells could be mediated through the p53 induction and CD95 (APO-1) signaling pathways.
American Psychological Association (APA)
Ng, Lean-Teik& Wu, Shu-Jing. 2011. Antiproliferative Activity of Cinnamomum cassia Constituents and Effects of Pifithrin-Alpha on Their Apoptotic Signaling Pathways in Hep G2 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-475934
Modern Language Association (MLA)
Ng, Lean-Teik& Wu, Shu-Jing. Antiproliferative Activity of Cinnamomum cassia Constituents and Effects of Pifithrin-Alpha on Their Apoptotic Signaling Pathways in Hep G2 Cells. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-475934
American Medical Association (AMA)
Ng, Lean-Teik& Wu, Shu-Jing. Antiproliferative Activity of Cinnamomum cassia Constituents and Effects of Pifithrin-Alpha on Their Apoptotic Signaling Pathways in Hep G2 Cells. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-475934
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475934