Automated Synthesis of 18F-Fluoropropoxytryptophan for Amino Acid Transporter System Imaging
Joint Authors
Shih, I-Hong
Duan, Xu-Dong
Yang, Kevin
Zhang, Yin-Han
Yang, David J.
Williams, Michael D.
Kong, Fan-Lin
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
This study was to develop a cGMP grade of [18F]fluoropropoxytryptophan (18F-FTP) to assess tryptophan transporters using an automated synthesizer.
Methods.
Tosylpropoxytryptophan (Ts-TP) was reacted with K18F/kryptofix complex.
After column purification, solvent evaporation, and hydrolysis, the identity and purity of the product were validated by radio-TLC (1M-ammonium acetate : methanol = 4 : 1) and HPLC (C-18 column, methanol : water = 7 : 3) analyses.
In vitro cellular uptake of 18F-FTP and 18F-FDG was performed in human prostate cancer cells.
PET imaging studies were performed with 18F-FTP and 18F-FDG in prostate and small cell lung tumor-bearing mice (3.7 MBq/mouse, iv).
Results.
Radio-TLC and HPLC analyses of 18F-FTP showed that the Rf and Rt values were 0.9 and 9 min, respectively.
Radiochemical purity was >99%.
The radiochemical yield was 37.7% (EOS 90 min, decay corrected).
Cellular uptake of 18F-FTP and 18F-FDG showed enhanced uptake as a function of incubation time.
PET imaging studies showed that 18F-FTP had less tumor uptake than 18F-FDG in prostate cancer model.
However, 18F-FTP had more uptake than 18F-FDG in small cell lung cancer model.
Conclusion.
18F-FTP could be synthesized with high radiochemical yield.
Assessment of upregulated transporters activity by 18F-FTP may provide potential applications in differential diagnosis and prediction of early treatment response.
American Psychological Association (APA)
Shih, I-Hong& Duan, Xu-Dong& Kong, Fan-Lin& Williams, Michael D.& Yang, Kevin& Zhang, Yin-Han…[et al.]. 2014. Automated Synthesis of 18F-Fluoropropoxytryptophan for Amino Acid Transporter System Imaging. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-475968
Modern Language Association (MLA)
Shih, I-Hong…[et al.]. Automated Synthesis of 18F-Fluoropropoxytryptophan for Amino Acid Transporter System Imaging. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-475968
American Medical Association (AMA)
Shih, I-Hong& Duan, Xu-Dong& Kong, Fan-Lin& Williams, Michael D.& Yang, Kevin& Zhang, Yin-Han…[et al.]. Automated Synthesis of 18F-Fluoropropoxytryptophan for Amino Acid Transporter System Imaging. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-475968
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475968