![](/images/graphics-bg.png)
Placing the RPL32 Promoter Upstream of a Second Promoter Results in a Strongly Increased Number of Stably Transfected Mammalian Cell Lines That Display High Protein Expression Levels
Joint Authors
Verhees, J. A.
Otte, A. P.
Siep, M.
Hamer, K.
Hoeksema, F.
Source
Biotechnology Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Information Technology and Computer Science
Abstract EN
The use of high stringency selection systems commonly results in a strongly diminished number of stably transfected mammalian cell lines.
Here we placed twelve different promoters upstream of an adjacent primary promoter and tested whether this might result in an increased number of colonies; this is in the context of a stringent selection system.
We found that only the promoter of the human ribosomal protein, RPL32, induced a high number of colonies in CHO-DG44 cells.
This phenomenon was observed when the RPL32 promoter was combined with the CMV, SV40, EF1-α, and the β-actin promoters.
In addition, these colonies displayed high protein expression levels.
The RPL32 promoter had to be functionally intact, since the deletion of a small region upstream of the transcription start site demolished its positive action.
We conclude that adding the RPL32 promoter to an expression cassette in cis may be a powerful tool to augment gene expression levels.
American Psychological Association (APA)
Hoeksema, F.& Hamer, K.& Siep, M.& Verhees, J. A.& Otte, A. P.. 2010. Placing the RPL32 Promoter Upstream of a Second Promoter Results in a Strongly Increased Number of Stably Transfected Mammalian Cell Lines That Display High Protein Expression Levels. Biotechnology Research International،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-476008
Modern Language Association (MLA)
Hoeksema, F.…[et al.]. Placing the RPL32 Promoter Upstream of a Second Promoter Results in a Strongly Increased Number of Stably Transfected Mammalian Cell Lines That Display High Protein Expression Levels. Biotechnology Research International No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-476008
American Medical Association (AMA)
Hoeksema, F.& Hamer, K.& Siep, M.& Verhees, J. A.& Otte, A. P.. Placing the RPL32 Promoter Upstream of a Second Promoter Results in a Strongly Increased Number of Stably Transfected Mammalian Cell Lines That Display High Protein Expression Levels. Biotechnology Research International. 2010. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-476008
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476008