![](/images/graphics-bg.png)
Cytotoxicity of Cricula triphenestrata Cocoon Extract on Human Fibroblasts
Joint Authors
Tontowi, Alva Edy
Sunarintyas, Siti
Siswomihardjo, Widowati
Source
International Journal of Biomaterials
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-05
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Objectives.
The aim of this paper was to evaluate the cytotoxicity of Indonesian silkworm cocoon extract of Cricula triphenestrata on human fibroblasts.
Methods and Materials.
The cocoon shells of the silkworm Cricula triphenestrata were degumming.
The shells were mixed with an aqueous solution of 0.3% Na2CO3 at 98°C for 1 hour.
The solution was then dialyzed in cellulose membranes against deionized water for 3 days.
The cocoon shells extract powder was collected via rotary evaporation and dried under freeze dryer.
Cell culture medium was exposed to Cricula triphenestrata cocoon extract (0.01–100 μg/mL) for 24 hours.
The primary human gingival fibroblasts were exposed to the treated cell culture medium for 24 hours.
Cytotoxicity evaluation was done by MTT method.
The data were analyzed by one-way ANOVA.
Result.
The result revealed no significant cytotoxicity of Cricula triphenestrata cocoon extract against human fibroblasts at a concentration up to 100 μg/mL (P>0.05).
Conclusion.
Cricula triphenestrata cocoon extract was not cytotoxic on human gingival fibroblast cells.
American Psychological Association (APA)
Sunarintyas, Siti& Siswomihardjo, Widowati& Tontowi, Alva Edy. 2012. Cytotoxicity of Cricula triphenestrata Cocoon Extract on Human Fibroblasts. International Journal of Biomaterials،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-476025
Modern Language Association (MLA)
Sunarintyas, Siti…[et al.]. Cytotoxicity of Cricula triphenestrata Cocoon Extract on Human Fibroblasts. International Journal of Biomaterials No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-476025
American Medical Association (AMA)
Sunarintyas, Siti& Siswomihardjo, Widowati& Tontowi, Alva Edy. Cytotoxicity of Cricula triphenestrata Cocoon Extract on Human Fibroblasts. International Journal of Biomaterials. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-476025
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476025