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Mitochondrial DNA Instability in Cells Lacking Aconitase Correlates with Iron Citrate Toxicity
Joint Authors
Farooq, Muhammad A.
Dong, Zhejun
Liu, Zhengchang
Xiao, Fei
Pracheil, Tammy M.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Aconitase, the second enzyme of the tricarboxylic acid cycle encoded by ACO1 in the budding yeast Saccharomyces cerevisiae, catalyzes the conversion of citrate to isocitrate.
aco1Δ results in mitochondrial DNA (mtDNA) instability.
It has been proposed that Aco1 binds to mtDNA and mediates its maintenance.
Here we propose an alternative mechanism to account for mtDNA loss in aco1Δ mutant cells.
We found that aco1Δ activated the RTG pathway, resulting in increased expression of genes encoding citrate synthase.
By deleting RTG1, RTG3, or genes encoding citrate synthase, mtDNA instability was prevented in aco1Δ mutant cells.
Increased activity of citrate synthase leads to iron accumulation in the mitochondria.
Mutations in MRS3 and MRS4, encoding two mitochondrial iron transporters, also prevented mtDNA loss due to aco1Δ.
Mitochondria are the main source of superoxide radicals, which are converted to H2O2 through two superoxide dismutases, Sod1 and Sod2.
H2O2 in turn reacts with Fe2+ to generate very active hydroxyl radicals.
We found that loss of Sod1, but not Sod2, prevents mtDNA loss in aco1Δ mutant cells.
We propose that mtDNA loss in aco1Δ mutant cells is caused by the activation of the RTG pathway and subsequent iron citrate accumulation and toxicity.
American Psychological Association (APA)
Farooq, Muhammad A.& Pracheil, Tammy M.& Dong, Zhejun& Xiao, Fei& Liu, Zhengchang. 2013. Mitochondrial DNA Instability in Cells Lacking Aconitase Correlates with Iron Citrate Toxicity. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-476065
Modern Language Association (MLA)
Farooq, Muhammad A.…[et al.]. Mitochondrial DNA Instability in Cells Lacking Aconitase Correlates with Iron Citrate Toxicity. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-476065
American Medical Association (AMA)
Farooq, Muhammad A.& Pracheil, Tammy M.& Dong, Zhejun& Xiao, Fei& Liu, Zhengchang. Mitochondrial DNA Instability in Cells Lacking Aconitase Correlates with Iron Citrate Toxicity. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-476065
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476065