Endophytic Fungal Diversity in Medicinal Plants of Western Ghats, India

Abstract EN

Endophytes constitute an important component of microbial diversity, and in the present investigation, seven plant species with rich ethnobotanical uses representing six families were analyzed for the presence of endophytic fungi from their natural habitats during monsoon (May/June) and winter (November/December) seasons of 2007.

Fungal endophytes were isolated from healthy plant parts such as stem, root, rhizome, and inflorescence employing standard isolation methods.

One thousand five hundred and twenty-nine fungal isolates were obtained from 5200 fragments.

Stem fragments harbored more endophytes (80.37%) than roots (19.22%).

31 fungal taxa comprised of coelomycetes (65%), hyphomycetes (32%), and ascomycetes (3%).

Fusarium, Acremonium, Colletotrichum, Chaetomium, Myrothecium, Phomopsis, and Pestalotiopsis spp.

were commonly isolated.

Diversity indices differed significantly between the seasons (P<0.001).

Species richness was greater for monsoon isolations than winter.

Host specificity was observed for few fungal endophytes.

UPGMA cluster analysis grouped the endophytes into distinct clusters on the basis of genetic distance.

This study is the first report on the diversity and host-specificity of endophytic fungal taxa were from the semi evergreen forest type in Talacauvery subcluster of Western Ghats.