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Mechanical Stress Stimulates the OsteoOdontoblastic Differentiation of Human Stem Cells from Apical Papilla via ERK 12 and JNK MAPK Pathways
Joint Authors
Mu, Jinquan
Lv, Taohong
Yu, Jinhua
Ma, Jie
Wang, Zilu
Ma, Shu
Liu, Jin
Mu, Chao
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background Information.
Stem cells from apical papilla (SCAPs) are a potent candidate for the apexogenesis/apexification due to their multiple differentiation capacity.
During the orthodontic treatment of developing teeth, SCAPs in vivo are usually subjected to the cyclic stress induced by compression forces.
However, it remains unclear whether mechanical stress can affect the proliferation and differentiation of human SCAPs.
Results.
Human SCAPs were isolated and stimulated by 200 g mechanical stimuli for 30 min and their proliferation and differentiation capacity were evaluated in vitro at different time points.
MTT and FCM results demonstrated that cell proliferation was enhanced, while TEM findings showed the morphological and ultrastructural changes in stress-treated SCAPs.
ALP activity and mineralization capacity of stress-treated SCAPs were upregulated .
In the meantime, higher odontogenic and osteogenic differentiation were found in stress-treated SCAPs by real-time RT-PCR and Western blot, as indicated by the expression of related markers at both mRNA and protein levels.
Moreover, the protein expressions of pJNK and pERK MAPK pathways were upregulated.
Conclusion.
Together, these findings suggest that mechanical stress is an important factor affecting the proliferation and differentiation of SCAPs via the activation of ERK and JNK signaling pathway.
American Psychological Association (APA)
Mu, Chao& Lv, Taohong& Wang, Zilu& Ma, Shu& Ma, Jie& Liu, Jin…[et al.]. 2014. Mechanical Stress Stimulates the OsteoOdontoblastic Differentiation of Human Stem Cells from Apical Papilla via ERK 12 and JNK MAPK Pathways. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-476145
Modern Language Association (MLA)
Mu, Chao…[et al.]. Mechanical Stress Stimulates the OsteoOdontoblastic Differentiation of Human Stem Cells from Apical Papilla via ERK 12 and JNK MAPK Pathways. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-476145
American Medical Association (AMA)
Mu, Chao& Lv, Taohong& Wang, Zilu& Ma, Shu& Ma, Jie& Liu, Jin…[et al.]. Mechanical Stress Stimulates the OsteoOdontoblastic Differentiation of Human Stem Cells from Apical Papilla via ERK 12 and JNK MAPK Pathways. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-476145
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476145