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Growth and Replication of Infectious Bursal Disease Virus in the DF-1 Cell Line and Chicken Embryo Fibroblasts
Joint Authors
Chung, Ill-Min
Thiruvengadam, Muthu
Rekha, Kaliyaperumal
Sivasubramanian, Chandran
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Infectious bursal disease virus (IBDV) causes a highly contagious disease in young chicks and leads to significant economic losses in the poultry industry.
To determine a suitable cell line for IBDV infection, replication, and growth kinetics of the virus, DF-1 cells and chicken embryo fibroblasts (CEF) were used.
The population doubling per day (Pd/D) was found to be higher in DF-1 as compared to CEF cells.
A suitable time of infection (TOI) was established for increased production of virus and greater infectivity titers.
The DF-1 and CEF cells were found to be susceptible to infection by producing marked cytopathic effects (CPEs), and the growth curves of IBDV in DF-1 and CEF cells were evaluated by infectivity assay using tissue culture infectious dose (TCID50).
The cytopathic effects of the virus in DF-1 and CEF cells were found to be similar, but higher viral titers were detected in the DF-1 cells as compared to CEF.
Thus the DF-1 cell line had a higher growth potential and infectivity, which will be of advantage in vaccine production.
American Psychological Association (APA)
Rekha, Kaliyaperumal& Sivasubramanian, Chandran& Chung, Ill-Min& Thiruvengadam, Muthu. 2014. Growth and Replication of Infectious Bursal Disease Virus in the DF-1 Cell Line and Chicken Embryo Fibroblasts. BioMed Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-476175
Modern Language Association (MLA)
Rekha, Kaliyaperumal…[et al.]. Growth and Replication of Infectious Bursal Disease Virus in the DF-1 Cell Line and Chicken Embryo Fibroblasts. BioMed Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-476175
American Medical Association (AMA)
Rekha, Kaliyaperumal& Sivasubramanian, Chandran& Chung, Ill-Min& Thiruvengadam, Muthu. Growth and Replication of Infectious Bursal Disease Virus in the DF-1 Cell Line and Chicken Embryo Fibroblasts. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-476175
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476175