NF-κB and Matrix-Dependent Regulation of Osteopontin Promoter Activity in Allylamine-Activated Vascular Smooth Muscle Cells
Joint Authors
Williams, E. Spencer
Wilson, Emily
Ramos, Kenneth S.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Repeated cycles of oxidative injury by allylamine in vivo induce a proliferative rat vascular (aortic) smooth muscle cell (vSMC) phenotype characterized by matrix-dependent enhancement of mitogenic sensitivity, changes in cell surface integrin expression, and osteopontin (opn) overexpression.
Here, we show that constitutive and mitogen-stimulated NF-κB DNA binding activity is enhanced in allylamine vSMCs.
Matrix-specific changes in cellular Rel protein expression were observed in allylamine vSMCs.
The NF-κB DNA binding element located at −1943 in the 5′-UTR strongly inhibited opn promoter activity in allylamine vSMCs, and this response was regulated by the extracellular matrix.
Constitutive increases in opn promoter activity were only seen when allylamine cells were seeded on a fibronectin substrate, and this response was independent of the NF-κB DNA binding sequence within the regulatory region.
Thus, NF-κB functions as a critical regulator of the allylamine-induced proliferative phenotype in vSMCs.
American Psychological Association (APA)
Williams, E. Spencer& Wilson, Emily& Ramos, Kenneth S.. 2012. NF-κB and Matrix-Dependent Regulation of Osteopontin Promoter Activity in Allylamine-Activated Vascular Smooth Muscle Cells. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-476323
Modern Language Association (MLA)
Williams, E. Spencer…[et al.]. NF-κB and Matrix-Dependent Regulation of Osteopontin Promoter Activity in Allylamine-Activated Vascular Smooth Muscle Cells. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-476323
American Medical Association (AMA)
Williams, E. Spencer& Wilson, Emily& Ramos, Kenneth S.. NF-κB and Matrix-Dependent Regulation of Osteopontin Promoter Activity in Allylamine-Activated Vascular Smooth Muscle Cells. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-476323
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476323