The Proteomic Changes in Cynara Cardunculus L. var. altilis DC Following the Etiolation Phenomena Using De Novo Sequence Analysis
Joint Authors
Addeo, Francesco
De Simone, Luciana
Chianese, Lina
Calabrese, Maria Grazia
Santoro, Simona
Caira, Simonetta
Lilla, Sergio
Guarino, Carmine
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-18
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Etiolation of vegetables is a complex phenomenon implying qualitative and quantitative protein changes.
The 2-DE protein profile of green and etiolated fleshy stalk samples has shown great differences in the 4–7 pH and 10 to 250 kDa mass range.
Currently, a shortage of Cynara DNA or protein sequences has required the use of de novo sequencing and BLAST similarity searches for protein identification.
Highlighted herein is an application of proteomics to the identification of proteins, which in a great part matched those from the Viridiplantae order.
Quantitative evaluation, statistical analyses, and MALDI-TOF MS characterization of the resolved spots in green and etiolated samples of C.
cardunculus enabled us to identify 46 out of 60 spots, containing 21 spots included conserved Viridiplantae domains.
The etiolation process is discussed in light of the identification of some proteins involved in specific biochemical and metabolic pathways.
American Psychological Association (APA)
Guarino, Carmine& De Simone, Luciana& Santoro, Simona& Caira, Simonetta& Lilla, Sergio& Calabrese, Maria Grazia…[et al.]. 2010. The Proteomic Changes in Cynara Cardunculus L. var. altilis DC Following the Etiolation Phenomena Using De Novo Sequence Analysis. Journal of Botany،Vol. 2010, no. 2010, pp.1-16.
https://search.emarefa.net/detail/BIM-476344
Modern Language Association (MLA)
Guarino, Carmine…[et al.]. The Proteomic Changes in Cynara Cardunculus L. var. altilis DC Following the Etiolation Phenomena Using De Novo Sequence Analysis. Journal of Botany No. 2010 (2010), pp.1-16.
https://search.emarefa.net/detail/BIM-476344
American Medical Association (AMA)
Guarino, Carmine& De Simone, Luciana& Santoro, Simona& Caira, Simonetta& Lilla, Sergio& Calabrese, Maria Grazia…[et al.]. The Proteomic Changes in Cynara Cardunculus L. var. altilis DC Following the Etiolation Phenomena Using De Novo Sequence Analysis. Journal of Botany. 2010. Vol. 2010, no. 2010, pp.1-16.
https://search.emarefa.net/detail/BIM-476344
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476344