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Immunogenicity of a Recombinant Influenza Virus Bearing Both the CD4+ and CD8+ T Cell Epitopes of Ovalbumin
Joint Authors
Garulli, Bruno
Castrucci, Maria R.
Kawaoka, Yoshihiro
Sciaraffia, Ester
Di Mario, Giuseppina
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Recombinant influenza viruses that bear the single immunodominant CD8+ T cell epitope OVA257−264 or the CD4+ T cell epitope OVA323−339 of the model antigen ovalbumin (OVA) have been useful tools in immunology.
Here, we generated a recombinant influenza virus, WSN-OVAI/II, that bears both OVA-specific CD8+ and CD4+ epitopes on its hemagglutinin molecule.
Live and heat-inactivated WSN-OVAI/II viruses were efficiently presented by dendritic cells in vitro to OT-I TCR transgenic CD8+ T cells and OT-II TCR transgenic CD4+ T cells.
In vivo, WSN-OVAI/II virus was attenuated in virulence, highly immunogenic, and protected mice from B16-OVA tumor challenge in a prophylactic model of vaccination.
Thus, WSN-OVAI/II virus represents an additional tool, along with OVA TCR transgenic mice, for further studies on T cell responses and may be of value in vaccine design.
American Psychological Association (APA)
Garulli, Bruno& Di Mario, Giuseppina& Sciaraffia, Ester& Kawaoka, Yoshihiro& Castrucci, Maria R.. 2011. Immunogenicity of a Recombinant Influenza Virus Bearing Both the CD4+ and CD8+ T Cell Epitopes of Ovalbumin. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-476388
Modern Language Association (MLA)
Garulli, Bruno…[et al.]. Immunogenicity of a Recombinant Influenza Virus Bearing Both the CD4+ and CD8+ T Cell Epitopes of Ovalbumin. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-476388
American Medical Association (AMA)
Garulli, Bruno& Di Mario, Giuseppina& Sciaraffia, Ester& Kawaoka, Yoshihiro& Castrucci, Maria R.. Immunogenicity of a Recombinant Influenza Virus Bearing Both the CD4+ and CD8+ T Cell Epitopes of Ovalbumin. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-476388
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476388