Species Classification and Quality Assessment of Cangzhu (Atractylodis Rhizoma) by High-Performance Liquid Chromatography and Chemometric Methods
Joint Authors
Xia, Yong-Gang
Wang, Di
Yang, Bing-You
Kuang, Hai-Xue
Wang, Qiu-Hong
Liang, Jun
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-25
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Fast and sensitive high-performance liquid chromatography (HPLC) coupled with chemometric methods was utilized to assist in the quality assessment of Cangzhu (Atractylodis Rhizoma).
By comparative analysis of chromatographic profiles, twelve common peaks were selected for multivariate analysis.
Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) of the chromatographic data demonstrated that 16 batches of Cangzhu samples could be welldifferentiated and categorized into two groups, which were closely related to their species (Atractylodes chinensis and A.
lancea).
By loading plots of PCA and OPLS-DA, the “common peaks” 2, 10, and 12 were defined as “marker peaks,” which were identified as atractylodinol, (4E,6E,12E)-tetradecatriene-8,10-diyne-1,3-diyl diacetate, and atractylodin, respectively.
These three “marker peaks” were then simultaneously quantified for further controlling the quality of Cangzhu, which showed acceptable linearity, both intraday and interday precisions (RSD ≤ 2.30%), repeatability (RSD ≤ 2.82%), and the recoveries of the three analytes in the range of 96.57–100.16%, with RSDs less than 1.46%.
Finally, linear discriminant analysis (LDA) was successfully used to build predictive models of the group membership based on the contents of three marker peaks.
Results of the present study demonstrated that HPLC-based metabolic profiling coupled with chemometric methods and quantificational determination was a very flexible, reliable, and effective way for homogeneity evaluation and quality assessment of traditional Chinese medicine.
American Psychological Association (APA)
Xia, Yong-Gang& Yang, Bing-You& Wang, Qiu-Hong& Liang, Jun& Wang, Di& Kuang, Hai-Xue. 2013. Species Classification and Quality Assessment of Cangzhu (Atractylodis Rhizoma) by High-Performance Liquid Chromatography and Chemometric Methods. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-476406
Modern Language Association (MLA)
Xia, Yong-Gang…[et al.]. Species Classification and Quality Assessment of Cangzhu (Atractylodis Rhizoma) by High-Performance Liquid Chromatography and Chemometric Methods. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-476406
American Medical Association (AMA)
Xia, Yong-Gang& Yang, Bing-You& Wang, Qiu-Hong& Liang, Jun& Wang, Di& Kuang, Hai-Xue. Species Classification and Quality Assessment of Cangzhu (Atractylodis Rhizoma) by High-Performance Liquid Chromatography and Chemometric Methods. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-476406
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476406