Caveolin 3, Flotillin 1 and Influenza Virus Hemagglutinin Reside in Distinct Domains on the Sarcolemma of Skeletal Myofibers
Joint Authors
Kaisto, Tuula
Kaakinen, Mika
Rahkila, Paavo
Metsikkö, Kalervo
Source
Biochemistry Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-05
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We examined the distribution of selected raft proteins on the sarcolemma of skeletal myofibers and the role of cholesterol environment in the distribution.
Immunofluorescence staining showed that flotillin-1 and influenza hemagglutinin exhibited rafts that located in the domains deficient of the dystrophin glycoprotein complex, but the distribution patterns of the two proteins were different.
Cholesterol depletion from the sarcolemma by means of methyl-β-cyclodextrin resulted in distorted caveolar morphology and redistribution of the caveolin 3 protein.
Concomitantly, the water permeability of the sarcolemma increased significantly.
However, cholesterol depletion did not reshuffle flotillin 1 or hemagglutinin.
Furthermore, a hemagglutinin variant that lacked a raft-targeting signals exhibited a similar distribution pattern as the native raft protein.
These findings indicate that each raft protein exhibits a strictly defined distribution in the sarcolemma.
Only the distribution of caveolin 3 that binds cholesterol was exclusively dependent on cholesterol environment.
American Psychological Association (APA)
Kaakinen, Mika& Kaisto, Tuula& Rahkila, Paavo& Metsikkö, Kalervo. 2012. Caveolin 3, Flotillin 1 and Influenza Virus Hemagglutinin Reside in Distinct Domains on the Sarcolemma of Skeletal Myofibers. Biochemistry Research International،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-476408
Modern Language Association (MLA)
Kaakinen, Mika…[et al.]. Caveolin 3, Flotillin 1 and Influenza Virus Hemagglutinin Reside in Distinct Domains on the Sarcolemma of Skeletal Myofibers. Biochemistry Research International No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-476408
American Medical Association (AMA)
Kaakinen, Mika& Kaisto, Tuula& Rahkila, Paavo& Metsikkö, Kalervo. Caveolin 3, Flotillin 1 and Influenza Virus Hemagglutinin Reside in Distinct Domains on the Sarcolemma of Skeletal Myofibers. Biochemistry Research International. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-476408
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476408