Functional Characterization of N-Terminally GFP-Tagged GLP-1 Receptor
Joint Authors
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-10-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The glucagon-like peptide-1 receptor (GLP-1 receptor) mediates important effects on peripheral tissues and the central nervous system.
It seems one of the most promising therapeutic targets for treatment of diabetes mellitus type 2.
Surprisingly, very little is known about the cellular mechanisms that regulate its function in vivo.
One of the approaches to study receptor dynamics, expression, or signaling is using GFP-tagged fluorescent proteins.
In this study, we synthesized and characterized N-terminally GFP-tagged GLP-1 (GFP-GLP-1) receptor in CHO cells.
We demonstrated that GFP-GLP-1 receptor is weakly expressed in the plasma membranes and is functionally coupled to adenylyl cyclase via heterotrimeric G-proteins, similarly as its wild type.
American Psychological Association (APA)
Bavec, Aljoša& Ličar, Alenka. 2009. Functional Characterization of N-Terminally GFP-Tagged GLP-1 Receptor. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-476468
Modern Language Association (MLA)
Bavec, Aljoša& Ličar, Alenka. Functional Characterization of N-Terminally GFP-Tagged GLP-1 Receptor. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-10.
https://search.emarefa.net/detail/BIM-476468
American Medical Association (AMA)
Bavec, Aljoša& Ličar, Alenka. Functional Characterization of N-Terminally GFP-Tagged GLP-1 Receptor. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-476468
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476468