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Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids
Joint Authors
Scordino, Agata
Barresi, Vincenza
Ganea, Constanta
Cuttone, Giacomo
Mocanu, Maria Magdalena
Musumeci, Francesco
Privitera, Simona
Baran, Irina
Cirrone, Giuseppe A. Pablo
Gulino, Marisa
Musso, Nicolò
Garaiman, Alexandru
Grasso, Rosaria
Condorelli, Daniele F.
Ursu, Ioan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-03
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Following previous work, we investigated in more detail the relationship between apoptosis and delayed luminescence (DL) in human leukemia Jurkat T cells under a wide variety of treatments.
We used menadione and hydrogen peroxide to induce oxidative stress and two flavonoids, quercetin, and epigallocatechin gallate, applied alone or in combination with menadione or H2O2.
62 MeV proton beams were used to irradiate cells under a uniform dose of 2 or 10 Gy, respectively.
We assessed apoptosis, cell cycle distributions, and DL.
Menadione, H2O2 and quercetin were potent inducers of apoptosis and DL inhibitors.
Quercetin decreased clonogenic survival and the NAD(P)H level in a dose-dependent manner.
Proton irradiation with 2 Gy but not 10 Gy increased the apoptotic rate.
However, both doses induced a substantial G2/M arrest.
Quercetin reduced apoptosis and prolonged the G2/M arrest induced by radiation.
DL spectroscopy indicated that proton irradiation disrupted the electron flow within Complex I of the mitochondrial respiratory chain, thus explaining the massive necrosis induced by 10 Gy of protons and also suggested an equivalent action of menadione and quercetin at the level of the Fe/S center N2, which may be mediated by their binding to a common site within Complex I, probably the rotenone-binding site.
American Psychological Association (APA)
Baran, Irina& Ganea, Constanta& Privitera, Simona& Scordino, Agata& Barresi, Vincenza& Musumeci, Francesco…[et al.]. 2012. Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-476538
Modern Language Association (MLA)
Baran, Irina…[et al.]. Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-14.
https://search.emarefa.net/detail/BIM-476538
American Medical Association (AMA)
Baran, Irina& Ganea, Constanta& Privitera, Simona& Scordino, Agata& Barresi, Vincenza& Musumeci, Francesco…[et al.]. Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-476538
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476538