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Improving the Diagnosis of Bloodstream Infections : PCR Coupled with Mass Spectrometry
Joint Authors
Martró, Elisa
Quesada, M. Dolores
Jordana-Lluch, Elena
Ausina, Vicente
Giménez, Montserrat
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The reference method for the diagnosis of bloodstream infections is blood culture followed by biochemical identification and antibiotic susceptibility testing of the isolated pathogen.
This process requires 48 to 72 hours.
The rapid administration of the most appropriate antimicrobial treatment is crucial for the survival of septic patients; therefore, a rapid method that enables diagnosis directly from analysis of a blood sample without culture is needed.
A recently developed platform that couples broad-range PCR amplification of pathogen DNA with electrospray ionization mass spectrometry (PCR/ESI-MS) has the ability to identify virtually any microorganism from direct clinical specimens.
To date, two clinical evaluations of the PCR/ESI-MS technology for the diagnosis of bloodstream infections from whole blood have been published.
Here we discuss them and describe recent improvements that result in an enhanced sensitivity.
Other commercially available assays for the molecular diagnosis of bloodstream infections from whole blood are also reviewed.
The use of highly sensitive molecular diagnostic methods in combination with conventional procedures could substantially improve the management of septic patients.
American Psychological Association (APA)
Jordana-Lluch, Elena& Giménez, Montserrat& Quesada, M. Dolores& Ausina, Vicente& Martró, Elisa. 2014. Improving the Diagnosis of Bloodstream Infections : PCR Coupled with Mass Spectrometry. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-476555
Modern Language Association (MLA)
Jordana-Lluch, Elena…[et al.]. Improving the Diagnosis of Bloodstream Infections : PCR Coupled with Mass Spectrometry. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-476555
American Medical Association (AMA)
Jordana-Lluch, Elena& Giménez, Montserrat& Quesada, M. Dolores& Ausina, Vicente& Martró, Elisa. Improving the Diagnosis of Bloodstream Infections : PCR Coupled with Mass Spectrometry. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-476555
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476555