Engineering Molecular Beacons for Intracellular Imaging
Joint Authors
Han, Da
Chen, Tao
Wu, Cuichen Sam
Yang, Chaoyong James
Tan, Weihong
You, Mingxu
Peng, Lu
Williams, Kathryn R.
Source
International Journal of Molecular Imaging
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Molecular beacons (MBs) represent a class of nucleic acid probes with unique DNA hairpin structures that specifically target complementary DNA or RNA.
The inherent “OFF” to “ON” signal transduction mechanism of MBs makes them promising molecular probes for real-time imaging of DNA/RNA in living cells.
However, conventional MBs have been challenged with such issues as false-positive signals and poor biostability in complex cellular matrices.
This paper describes the novel engineering steps used to improve the fluorescence signal and reduce to background fluorescence, as well as the incorporation of unnatural nucleotide bases to increase the resistance of MBs to nuclease degradation for application in such fields as chemical analysis, biotechnology, and clinical medicine.
The applications of these de novo MBs for single-cell imaging will be also discussed.
American Psychological Association (APA)
Wu, Cuichen Sam& Peng, Lu& You, Mingxu& Han, Da& Chen, Tao& Williams, Kathryn R.…[et al.]. 2012. Engineering Molecular Beacons for Intracellular Imaging. International Journal of Molecular Imaging،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-476587
Modern Language Association (MLA)
Wu, Cuichen Sam…[et al.]. Engineering Molecular Beacons for Intracellular Imaging. International Journal of Molecular Imaging No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-476587
American Medical Association (AMA)
Wu, Cuichen Sam& Peng, Lu& You, Mingxu& Han, Da& Chen, Tao& Williams, Kathryn R.…[et al.]. Engineering Molecular Beacons for Intracellular Imaging. International Journal of Molecular Imaging. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-476587
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476587