Identification of Target Genes Involved in the Antiproliferative Effect of Enzyme-Modified Ginseng Extract in HepG2 Hepatocarcinoma Cell
Joint Authors
Lee, Yeon-Weol
Jang, Sung-Il
Cho, Chong-Kwan
Yoo, Hwa-Seung
Jang, Jun-Hyeog
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Ginsenosides are ginseng saponins, which are the major biologically active components of Panax ginseng, often metabolized by intestinal bacteria into more effective forms.
In this study, we found that the antiproliferative activity of ginseng increased after enzymatic processing of ginseng saponin (50% inhibitory concentration [IC50], >30 μg/mL), which may be the result of the accumulation of minor saponins, such as Rh1, Rg3, compound K, and PPT constituents in ginseng saponin.
Using the Agilent PrimeView Human Gene Expression Array, we found that the expression of several genes involved in apoptosis (caspase-4, Annexin A2, HSPA9, AIFM1, UQCRC2, and caspase-7) were increased in HepG2 human hepatocarcinoma cells after their treatment with enzyme-modified ginseng extract (EMGE).
Furthermore, several genes implicated in cell cycle progression (CDCA3, CDCA8, CABLES2, CDC25B, CNNM3, and CCNK) showed decreased expression in HepG2 cells treated with EMGE.
Finally, from flow cytometric analysis, we found that EMGE-treated HepG2 cells showed increased apoptotic sub-G1 population (24%), compared with that observed in DMSO-treated control cells (1.6%).
Taken together, our results suggest that EMGE induces anticancer activity through the induction of apoptosis-related genes and cell cycle arrest via decreased expression of cell cycle regulatory genes.
American Psychological Association (APA)
Jang, Sung-Il& Lee, Yeon-Weol& Cho, Chong-Kwan& Yoo, Hwa-Seung& Jang, Jun-Hyeog. 2013. Identification of Target Genes Involved in the Antiproliferative Effect of Enzyme-Modified Ginseng Extract in HepG2 Hepatocarcinoma Cell. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-476701
Modern Language Association (MLA)
Jang, Sung-Il…[et al.]. Identification of Target Genes Involved in the Antiproliferative Effect of Enzyme-Modified Ginseng Extract in HepG2 Hepatocarcinoma Cell. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-476701
American Medical Association (AMA)
Jang, Sung-Il& Lee, Yeon-Weol& Cho, Chong-Kwan& Yoo, Hwa-Seung& Jang, Jun-Hyeog. Identification of Target Genes Involved in the Antiproliferative Effect of Enzyme-Modified Ginseng Extract in HepG2 Hepatocarcinoma Cell. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-476701
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476701