Studies on the Anti-Inflammatory Effect and Its Mechanisms of Sophoridine
Joint Authors
Shen, Lianzhong
Li, Bo
Huang, Xiumei
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-09
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
This work is to study the anti-inflammatory effect and its mechanisms of sophoridine in vitro and in vivo.
For this aim, the influences of sophoridine on several inflammatory mediators were investigated.
Excessive inflammatory response in vitro model was developed by using lipopolysaccharide (LPS) to stimulate the mouse peritoneal macrophages and HL-60 cells to produce IL-6 and IL-8.
Carrageenin-induced mouse paw edema model was used as inflammatory response in vivo model.
MTT method, ultraviolet spectrophotometric method, and radioimmunoassay were used to measure the changes of TNFα, IL-6, PGE2, and IL-8 in in vitro cell culture supernatant or in the local inflammatory exudates.
The results showed that sophoridine inhibited the production of IL-8 in in vitro cell culture supernatant and inhibited the production of TNFα, PGE2, and IL-8 in the local inflammatory exudates but had no significant effects on the production of IL-6 in vitro and in vivo.
It is demonstrated that sophoridine’s anti-inflammatory effect was due to its ability to inhibit the production of cytokine and inflammatory mediators.
American Psychological Association (APA)
Huang, Xiumei& Li, Bo& Shen, Lianzhong. 2014. Studies on the Anti-Inflammatory Effect and Its Mechanisms of Sophoridine. Journal of Analytical Methods in Chemistry،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-476705
Modern Language Association (MLA)
Huang, Xiumei…[et al.]. Studies on the Anti-Inflammatory Effect and Its Mechanisms of Sophoridine. Journal of Analytical Methods in Chemistry No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-476705
American Medical Association (AMA)
Huang, Xiumei& Li, Bo& Shen, Lianzhong. Studies on the Anti-Inflammatory Effect and Its Mechanisms of Sophoridine. Journal of Analytical Methods in Chemistry. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-476705
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476705