High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride after In Vitro Transdermal Diffusion Studies
Joint Authors
Femenía-Font, Andrés
Merino, Virginia
Balaguer-Fernández, Cristina
del Rio-Sancho, Sergio
Serna-Jiménez, César E.
López-Castellano, Alicia
Calatayud-Pascual, M. Aracely
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The purpose of the present work was to validate an accurate and precise high-performance liquid chromatography (HPLC) method involving ultraviolet detection for the quantitative analysis of memantine hydrochloride.
In order to analyze a molecule with no chromophoric groups that could be detected by a UV/visible detector, it was necessary to extract the drug and to perform a dansylation reaction that enabled the UV/visible detection of the derivatized molecule.
Separation was carried out with a 150 mm Kromasil C18 column at room temperature.
The detection response, at 218 nm, was found to be linear in the concentration range from 0.5 to 50 μg/mL.
The method was validated for specificity, linearity, precision, accuracy, limit of detection, limit of quantification, and robustness.
The limit of detection (LOD) was 0.144 μg/mL, and the limit of quantification (LOQ) was 0.437 μg/mL.
The dansylated memantine complex was stable for at least five days in all the conditions evaluated.
The potential use of this method has been demonstrated by the quantification of memantine hydrochloride contained in samples from the study of its in vitro transdermal permeation.
American Psychological Association (APA)
del Rio-Sancho, Sergio& Serna-Jiménez, César E.& Calatayud-Pascual, M. Aracely& Balaguer-Fernández, Cristina& Femenía-Font, Andrés& Merino, Virginia…[et al.]. 2012. High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride after In Vitro Transdermal Diffusion Studies. Journal of Chemistry،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-476709
Modern Language Association (MLA)
del Rio-Sancho, Sergio…[et al.]. High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride after In Vitro Transdermal Diffusion Studies. Journal of Chemistry No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-476709
American Medical Association (AMA)
del Rio-Sancho, Sergio& Serna-Jiménez, César E.& Calatayud-Pascual, M. Aracely& Balaguer-Fernández, Cristina& Femenía-Font, Andrés& Merino, Virginia…[et al.]. High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride after In Vitro Transdermal Diffusion Studies. Journal of Chemistry. 2012. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-476709
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476709