Expanding the Use of a Fluorogenic Method to Determine Activity and Mode of Action of Bacillus thuringiensis Bacteriocins Against Gram-Positive and Gram-Negative Bacteria
Joint Authors
Barboza-Corona, Jose Eleazar
Pacheco Cano, R. D.
Espino Monzón, A. N.
Balagurusamy, N.
Salcedo-Hernández, Rubén
de la Fuente-Salcido, Norma M.
Bideshi, Dennis K.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Previously we described a rapid fluorogenic method to measure the activity of five bacteriocins produced by Mexican strains of Bacillus thuringiensis against B.
cereus 183.
Here we standardize this method to efficiently determine the activity of bacteriocins against both Gram-positive and Gram-negative bacteria.
It was determined that the crucial parameter required to obtain reproducible results was the number of cells used in the assay, that is, ~4 × 108 cell/mL and ~7 × 108 cell/mL, respectively, for target Gram-positive and Gram-negative bacteria.
Comparative analyses of the fluorogenic and traditional well-diffusion assays showed correlation coefficients of 0.88 to 0.99 and 0.83 to 0.99, respectively, for Gram-positive and Gram-negative bacteria.
The fluorogenic method demonstrated that the five bacteriocins of B.
thuringiensis have bacteriolytic and bacteriostatic activities against all microorganisms tested, including clinically significant bacteria such as Listeria monocytogenes, Proteus vulgaris, and Shigella flexneri reported previously to be resistant to the antimicrobials as determined using the well-diffusion protocol.
These results demonstrate that the fluorogenic assay is a more sensitive, reliable, and rapid method when compared with the well-diffusion method and can easily be adapted in screening protocols for bacteriocin production by other microorganisms.
American Psychological Association (APA)
de la Fuente-Salcido, Norma M.& Barboza-Corona, Jose Eleazar& Espino Monzón, A. N.& Pacheco Cano, R. D.& Balagurusamy, N.& Bideshi, Dennis K.…[et al.]. 2012. Expanding the Use of a Fluorogenic Method to Determine Activity and Mode of Action of Bacillus thuringiensis Bacteriocins Against Gram-Positive and Gram-Negative Bacteria. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-476776
Modern Language Association (MLA)
de la Fuente-Salcido, Norma M.…[et al.]. Expanding the Use of a Fluorogenic Method to Determine Activity and Mode of Action of Bacillus thuringiensis Bacteriocins Against Gram-Positive and Gram-Negative Bacteria. The Scientific World Journal No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-476776
American Medical Association (AMA)
de la Fuente-Salcido, Norma M.& Barboza-Corona, Jose Eleazar& Espino Monzón, A. N.& Pacheco Cano, R. D.& Balagurusamy, N.& Bideshi, Dennis K.…[et al.]. Expanding the Use of a Fluorogenic Method to Determine Activity and Mode of Action of Bacillus thuringiensis Bacteriocins Against Gram-Positive and Gram-Negative Bacteria. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-476776
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476776