Infiltration of Proinflammatory M1 Macrophages into the Outer Retina Precedes Damage in a Mouse Model of Age-Related Macular Degeneration
Joint Authors
Sene, Abdoulaye
Echegaray, Jose J.
Hollyfield, Joe G.
Ballmick, Asha
Huang, DeQiang
Betancourt, Michel
Perez, Victor L.
Ramkhellawan, Ghansham C.
Viteri, Eduardo
Ewald, Eric
Apte, Rajendra S.
Wen, Rong
Salomon, Robert G.
Feuer, William
Saeed, Ali M.
Tan, Yaohong
Hong, Li
Wang, Hua
Duffort, Stephanie
Laird, James M.
Cruz-Guilloty, Fernando
Source
International Journal of Inflammation
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-07
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Age-related macular degeneration (AMD) is a major cause of blindness in the developed world.
Oxidative stress and inflammation are implicated in AMD, but precise mechanisms remain poorly defined.
Carboxyethylpyrrole (CEP) is an AMD-associated lipid peroxidation product.
We previously demonstrated that mice immunized with CEP-modified albumin developed AMD-like degenerative changes in the outer retina.
Here, we examined the kinetics of lesion development in immunized mice and the presence of macrophages within the interphotoreceptor matrix (IPM), between the retinal pigment epithelium and photoreceptor outer segments.
We observed a significant and time-dependent increase in the number of macrophages in immunized mice relative to young age-matched controls prior to overt pathology.
These changes were more pronounced in BALB/c mice than in C57BL/6 mice.
Importantly, IPM-infiltrating macrophages were polarized toward the M1 phenotype but only in immunized mice.
Moreover, when Ccr2-deficient mice were immunized, macrophages were not present in the IPM and no retinal lesions were observed, suggesting a deleterious role for these cells in our model.
This work provides mechanistic evidence linking immune responses against oxidative damage with the presence of proinflammatory macrophages at sites of future AMD and experimentally demonstrates that manipulating immunity may be a target for modulating the development of AMD.
American Psychological Association (APA)
Cruz-Guilloty, Fernando& Saeed, Ali M.& Echegaray, Jose J.& Duffort, Stephanie& Ballmick, Asha& Tan, Yaohong…[et al.]. 2013. Infiltration of Proinflammatory M1 Macrophages into the Outer Retina Precedes Damage in a Mouse Model of Age-Related Macular Degeneration. International Journal of Inflammation،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-476814
Modern Language Association (MLA)
Cruz-Guilloty, Fernando…[et al.]. Infiltration of Proinflammatory M1 Macrophages into the Outer Retina Precedes Damage in a Mouse Model of Age-Related Macular Degeneration. International Journal of Inflammation No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-476814
American Medical Association (AMA)
Cruz-Guilloty, Fernando& Saeed, Ali M.& Echegaray, Jose J.& Duffort, Stephanie& Ballmick, Asha& Tan, Yaohong…[et al.]. Infiltration of Proinflammatory M1 Macrophages into the Outer Retina Precedes Damage in a Mouse Model of Age-Related Macular Degeneration. International Journal of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-476814
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476814