Application of a Simple In-House PCR-SSP Technique for HLA-B* 27 Typing in Spondyloarthritis Patients
Joint Authors
Rajadhyaksha, Anjali
Parasannanavar, Devaraj J.
Ghosh, Kanjaksha
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-19
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Background.
Microlymphocytotoxicity (MLCT) and flowcytometry (FC) are the conventional serological methods to detect HLA-B* 27.
Due to some disadvantages in these methods, most of the HLA laboratories have now switched over to molecular methods.
Molecular techniques based on commercial kits are expensive; as such many laboratories with limited funds in developing countries cannot afford these techniques.
Aims.
Our main aim was to standardize a simple inexpensive in-house PCR-SSP technique for HLA-B* 27 typing.
Materials and Methods.
Sequence Specific primers were designed to amplify all the subtypes of B* 27 using IMGT-HLA sequence database.
Accuracy was checked by retyping of 90 PCR-SSOP typed controls.
Results.
The presence of 149 bp specific band with control band on 2% agarose gel showed B* 27 positivity.
No discrepancies were found when compared with PCR-SSOP results.
The frequency of HLA-B* 27 was found to be significantly increased (68.75% versus 4.40%, O.R 46.909: P value 6.62E-32) among 700 SpA patients as compared to controls.
Clinically, 54% of patients had polyarticular arthritis with SI joints involvement (68%) and restricted spine flexion (60%).
Conclusion.
In-house PCR-SSP technique is very simple and inexpensive technique to detect B* 27 allele, which was strongly associated with SpA patients from Western India.
American Psychological Association (APA)
Parasannanavar, Devaraj J.& Rajadhyaksha, Anjali& Ghosh, Kanjaksha. 2013. Application of a Simple In-House PCR-SSP Technique for HLA-B* 27 Typing in Spondyloarthritis Patients. Arthritis،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-476850
Modern Language Association (MLA)
Parasannanavar, Devaraj J.…[et al.]. Application of a Simple In-House PCR-SSP Technique for HLA-B* 27 Typing in Spondyloarthritis Patients. Arthritis No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-476850
American Medical Association (AMA)
Parasannanavar, Devaraj J.& Rajadhyaksha, Anjali& Ghosh, Kanjaksha. Application of a Simple In-House PCR-SSP Technique for HLA-B* 27 Typing in Spondyloarthritis Patients. Arthritis. 2013. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-476850
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476850