Assessment of the Microbiota in Microdissected Tissues of Crohn's Disease Patients
Joint Authors
Vermeire, Severine
Van Assche, Gert
Rutgeerts, Paul
Lemmens, Bart
Sagaert, Xavier
De Hertogh, Gert
de Hoogt, Ronald
Joossens, Marie
Verhasselt, Peter
Aerssens, Jeroen
Source
International Journal of Inflammation
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The microbiota of the gastrointestinal tract is frequently mentioned as one of the key players in the etiopathogenesis of Crohn's disease (CD).
Four hypotheses have been suggested: the single, still unknown bacterial pathogen, an abnormal overall composition of the bowel microbiota (“dysbiosis”), an abnormal immunological reaction to an essentially normally composed microbiota, and increased bacterial translocation.
We propose that laser capture microdissection of selected microscopic structures, followed by broad-range 16S rRNA gene sequencing, is an excellent method to assess spatiotemporal alterations in the composition of the bowel microbiota in CD.
Using this approach, we demonstrated significant changes of the composition, abundance, and location of the gut microbiome in this disease.
Some of these abnormal findings persisted even after macroscopic mucosal healing.
Further investigations along these lines may lead to a better understanding of the possible involvement of the bowel bacteria in the development of clinical Crohn's disease.
American Psychological Association (APA)
De Hertogh, Gert& Lemmens, Bart& Verhasselt, Peter& de Hoogt, Ronald& Sagaert, Xavier& Joossens, Marie…[et al.]. 2011. Assessment of the Microbiota in Microdissected Tissues of Crohn's Disease Patients. International Journal of Inflammation،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-476980
Modern Language Association (MLA)
De Hertogh, Gert…[et al.]. Assessment of the Microbiota in Microdissected Tissues of Crohn's Disease Patients. International Journal of Inflammation No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-476980
American Medical Association (AMA)
De Hertogh, Gert& Lemmens, Bart& Verhasselt, Peter& de Hoogt, Ronald& Sagaert, Xavier& Joossens, Marie…[et al.]. Assessment of the Microbiota in Microdissected Tissues of Crohn's Disease Patients. International Journal of Inflammation. 2011. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-476980
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-476980