Ultrastructure and Light Microscope Analysis of Intact Skin after a Varying Number of Low Level Laser Irradiations in Mice
Joint Authors
Camargo Rizzi, Élen
Watanabe, Ii-sei
Iyomasa, Daniela Mizusaki
Caroline Leão, Juliane
Issa, João Paulo Mardegan
Iyomasa, Mamie Mizusaki
Dias, Fernando José
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-29
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Low level laser therapy (LLLT) has been used to relieve pain, inflammation, and wound healing processes.
Thus, the skin is overexposed to laser and this effect is not completely understood.
This study analyzed the effects of the number of laser applications (three, six, and 10) on the intact skin of the masseteric region in mice of strain HRS/J.
The animals (n=30) were equally divided into control (0 J/cm2) and irradiated (20 J/cm2), and each of these groups was further equally divided according to the number of laser applications (three, six, and 10) and underwent LLLT on alternate days.
Samples were analyzed by light microscopy and transmission electron microscope (TEM).
The animals receiving applications exhibited open channels more dilated between the keratinocytes and photobiomodulation effect on endothelial cells and fibroblasts by TEM.
Under the light microscope after 10 laser applications, the type I collagen decreased (P<0.05) compared to the three and six applications.
Under these experimental conditions, all numbers of applications provided photobiomodulatory effect on the epidermis and dermis, without damage.
More studies are needed to standardize the energy density and number of applications recommended for laser therapy to have a better cost-benefit ratio associated with treatment.
American Psychological Association (APA)
Iyomasa, Mamie Mizusaki& Caroline Leão, Juliane& Camargo Rizzi, Élen& Issa, João Paulo Mardegan& Dias, Fernando José& Watanabe, Ii-sei…[et al.]. 2014. Ultrastructure and Light Microscope Analysis of Intact Skin after a Varying Number of Low Level Laser Irradiations in Mice. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-477014
Modern Language Association (MLA)
Iyomasa, Mamie Mizusaki…[et al.]. Ultrastructure and Light Microscope Analysis of Intact Skin after a Varying Number of Low Level Laser Irradiations in Mice. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-477014
American Medical Association (AMA)
Iyomasa, Mamie Mizusaki& Caroline Leão, Juliane& Camargo Rizzi, Élen& Issa, João Paulo Mardegan& Dias, Fernando José& Watanabe, Ii-sei…[et al.]. Ultrastructure and Light Microscope Analysis of Intact Skin after a Varying Number of Low Level Laser Irradiations in Mice. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-477014
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477014