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Leaching of Mycobacterium avium subsp. paratuberculosis in Soil under In Vitro Conditions
Joint Authors
Lin, Tsang L.
Wu, Ching C.
Negron, Maria
Habteselassie, Mussie Y.
Raizman, Eran A.
Turco, Ronald F.
Source
Veterinary Medicine International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-13
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Mycobacterium avium subsp paratuberculosis (Map), the causative agent of Johne's disease, has a robust ability to survive in the environment.
However, the ability of Map to migrate through soil to drainage tiles or ground water, leave the farm, and leak into local watersheds is inadequately documented.
In order to assess the ability of Map to leach through soil, two laboratory experiments were conducted.
In the first study, 8 columns (30 cm long each) of a sandy loam soil were treated with pure cultures of Map.
Two soil moisture levels and two Map concentrations were used.
The columns were leached with 500 mL of water once a week for three weeks, the leachate was collected, and detection analysis was conducted.
In the second experiment, manure from Map negative cows (control) and Map high shedder cows (treatment) were deposited on 8 similar columns and the columns were leached with 500 mL of water once a week for four weeks.
Map detection and numeration in leachate samples were done with RT-PCR and culture techniques, respectively.
Using RT-PCR, Map could be detected in the leachates in both experiments for several weeks but could only be recovered using culture techniques in experiment one.
Combined, these experiments indicate the potential for Map to move through soil as a result of rainfall or irrigation following application.
American Psychological Association (APA)
Raizman, Eran A.& Habteselassie, Mussie Y.& Wu, Ching C.& Lin, Tsang L.& Negron, Maria& Turco, Ronald F.. 2011. Leaching of Mycobacterium avium subsp. paratuberculosis in Soil under In Vitro Conditions. Veterinary Medicine International،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-477038
Modern Language Association (MLA)
Raizman, Eran A.…[et al.]. Leaching of Mycobacterium avium subsp. paratuberculosis in Soil under In Vitro Conditions. Veterinary Medicine International No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-477038
American Medical Association (AMA)
Raizman, Eran A.& Habteselassie, Mussie Y.& Wu, Ching C.& Lin, Tsang L.& Negron, Maria& Turco, Ronald F.. Leaching of Mycobacterium avium subsp. paratuberculosis in Soil under In Vitro Conditions. Veterinary Medicine International. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-477038
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477038