Troglitazone Induces Extracellular Matrix and Cytoskeleton Remodeling in Mouse Collecting Duct Cells
Joint Authors
Clark, Jordan
Corinaldi, Jaime
Jasmin, Bernard J.
Nasrallah, Rania
Miura, Pedro
Paris, Geneviève
Hébert, Richard L.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Peroxisome proliferator-activated receptor (PPARγ) has been shown to have a protective role in the nephron through its ability to inhibit a transforming growth factor- (TGF-β) mediated fibrotic response.
In contrast, PPARγ was also shown to induce a mesenchymal transformation in epithelial intestinal cells.
A fibrotic response in the collecting duct has only recently been established; however, the entire collecting duct has not been fully examined.
Inner medullary collecting duct cells (IMCD-K2) and mouse cortical collecting duct cells (M1), representing the cortical and medullary collecting duct, were exposed to 5–10 μM troglitazone for 24 hours.
Troglitazone resulted in an elongated morphology, 60% decreases in E-cadherin and β-catenin, a 35% decrease in α-catenin, and a 1.5-fold increase in fibronectin.
These effects were not reversed with PPARγ antagonists or affected with PPARγ overexpression.
Our results indicate that troglitazone induced a mesenchymal-like transformation in M1 and IMCD-K2 epithelial cells independently of PPARγ.
American Psychological Association (APA)
Corinaldi, Jaime& Nasrallah, Rania& Clark, Jordan& Paris, Geneviève& Miura, Pedro& Jasmin, Bernard J.…[et al.]. 2012. Troglitazone Induces Extracellular Matrix and Cytoskeleton Remodeling in Mouse Collecting Duct Cells. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-477106
Modern Language Association (MLA)
Corinaldi, Jaime…[et al.]. Troglitazone Induces Extracellular Matrix and Cytoskeleton Remodeling in Mouse Collecting Duct Cells. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-477106
American Medical Association (AMA)
Corinaldi, Jaime& Nasrallah, Rania& Clark, Jordan& Paris, Geneviève& Miura, Pedro& Jasmin, Bernard J.…[et al.]. Troglitazone Induces Extracellular Matrix and Cytoskeleton Remodeling in Mouse Collecting Duct Cells. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-477106
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477106