Molecular Characterization and SNP Detection of CD14 Gene of Crossbred Cattle
Joint Authors
Chakravarty, A. K.
Chatterjee, P. N.
Pal, Aruna
Sharma, Arjava
Bhattacharya, T. K.
Source
Molecular Biology International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-25
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
CD14 is an important molecule for innate immunity that can act against a wide range of pathogens.
The present paper has characterized CD14 gene of crossbred (CB) cattle (Bos indicus×Bos taurus).
Cloning and sequence analysis of CD14 cDNA revealed 1119 nucleotide long open reading frame encoding 373 amino acids protein and 20 amino acids signal peptide.
CB cattle CD14 gene exhibited a high percentage of nucleotide identity (59.3–98.1%) with the corresponding mammalian homologs.
Cattle and buffalo appear to have diverged from a common ancestor in phylogenetic analysis.
25 SNPs with 17 amino acid changes were newly reported and the site for mutational hot-spot was detected in CB cattle CD14 gene.
Non-synonymous substitutions exceeding synonymous substitutions indicate the evolution of this protein through positive selection among domestic animals.
Predicted protein structures obtained from deduced amino acid sequence indicated CB cattle CD14 molecule to be a receptor with horse shoe-shaped structure.
The sites for LPS binding, LPS signalling, leucine-rich repeats, putative N-linked glycosylation, O-linked glycosylation, glycosyl phosphatidyl inositol anchor, disulphide bridges, alpha helix, beta strand, leucine rich nuclear export signal, leucine zipper and domain linker were predicted.
Most of leucine and cysteine residues remain conserved across the species.
American Psychological Association (APA)
Pal, Aruna& Sharma, Arjava& Bhattacharya, T. K.& Chatterjee, P. N.& Chakravarty, A. K.. 2011. Molecular Characterization and SNP Detection of CD14 Gene of Crossbred Cattle. Molecular Biology International،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-477136
Modern Language Association (MLA)
Pal, Aruna…[et al.]. Molecular Characterization and SNP Detection of CD14 Gene of Crossbred Cattle. Molecular Biology International No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-477136
American Medical Association (AMA)
Pal, Aruna& Sharma, Arjava& Bhattacharya, T. K.& Chatterjee, P. N.& Chakravarty, A. K.. Molecular Characterization and SNP Detection of CD14 Gene of Crossbred Cattle. Molecular Biology International. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-477136
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477136