Antimetastatic Potentials of Dioscorea nipponica on Melanoma In Vitro and In Vivo
Joint Authors
Ho, Mao-Lin
Chen, Kuo-Shuen
Chen, Pei-Ni
Chen, Jia-Jing
Hsieh, Yih-Shou
Lin, Shu-Jiuan
Chen, Jia-Yuh
Kuo, Wu-Hsien
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-01-10
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Recent studies have revealed pleiotropic anticancer and antiproliferative capabilities of Dioscorea nipponica Makino whereas the effect of this plant on metastasis of cancer cells has not been clearly clarified.
In the present study, we extracted Dioscorea nipponica Makino with methanol (DNE1), chloroform (DNE2), ethyl acetate (DNE3), n-butanol (DNE4), and water (DNE5).
We first demonstrate that DNE3 was found to be effective in reducing the lung metastases formation by about 99.5% as compared to vehicle-treated control animals.
When a nontoxic concentration of the extract was treated directly to highly metastatic murin melanoma cells (B16F10) and human melanoma cells (A2058) in vitro, it exerted a dose-dependent inhibitory effect on the invasion (P<.001), motility (P<.001), secretion of MMPs (P<.001), and u-PA (P<.001) of both cell lines.
To investigate the possible mechanisms involved in these events, we performed western blot analysis to find that DNE inhibited phosphorylation of Akt.
A treatment with DNE3 to B16F10 cells also inhibited the activation of NF-κB and increased the expression of IkappaB.
Taken together, these findings suggested that DNE3 could reduce the metastasis of melanoma cells, thereby constituting an adjuvant treatment for metastasis control.
American Psychological Association (APA)
Ho, Mao-Lin& Hsieh, Yih-Shou& Chen, Jia-Yuh& Chen, Kuo-Shuen& Chen, Jia-Jing& Kuo, Wu-Hsien…[et al.]. 2011. Antimetastatic Potentials of Dioscorea nipponica on Melanoma In Vitro and In Vivo. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-477187
Modern Language Association (MLA)
Ho, Mao-Lin…[et al.]. Antimetastatic Potentials of Dioscorea nipponica on Melanoma In Vitro and In Vivo. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-477187
American Medical Association (AMA)
Ho, Mao-Lin& Hsieh, Yih-Shou& Chen, Jia-Yuh& Chen, Kuo-Shuen& Chen, Jia-Jing& Kuo, Wu-Hsien…[et al.]. Antimetastatic Potentials of Dioscorea nipponica on Melanoma In Vitro and In Vivo. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-477187
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477187