Effects of Substrate Mechanics on Contractility of Cardiomyocytes Generated from Human Pluripotent Stem Cells
Joint Authors
Hazeltine, Laurie B.
Crone, Wendy C.
Wakatsuki, Tetsuro
Delgado, Stephanie M.
Palecek, Sean P.
Badur, Mehmet G.
Simmons, Chelsey S.
Salick, Max R.
Han, Wenqing
Lian, Xiaojun
Pruitt, Beth L.
Source
International Journal of Cell Biology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-09
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Human pluripotent stem cell (hPSC-) derived cardiomyocytes have potential applications in drug discovery, toxicity testing, developmental studies, and regenerative medicine.
Before these cells can be reliably utilized, characterization of their functionality is required to establish their similarity to native cardiomyocytes.
We tracked fluorescent beads embedded in 4.4–99.7 kPa polyacrylamide hydrogels beneath contracting neonatal rat cardiomyocytes and cardiomyocytes generated from hPSCs via growth-factor-induced directed differentiation to measure contractile output in response to changes in substrate mechanics.
Contraction stress was determined using traction force microscopy, and morphology was characterized by immunocytochemistry for α-actinin and subsequent image analysis.
We found that contraction stress of all types of cardiomyocytes increased with substrate stiffness.
This effect was not linked to beating rate or morphology.
We demonstrated that hPSC-derived cardiomyocyte contractility responded appropriately to isoprenaline and remained stable in culture over a period of 2 months.
This study demonstrates that hPSC-derived cardiomyocytes have appropriate functional responses to substrate stiffness and to a pharmaceutical agent, which motivates their use in further applications such as drug evaluation and cardiac therapies.
American Psychological Association (APA)
Hazeltine, Laurie B.& Simmons, Chelsey S.& Salick, Max R.& Lian, Xiaojun& Badur, Mehmet G.& Han, Wenqing…[et al.]. 2012. Effects of Substrate Mechanics on Contractility of Cardiomyocytes Generated from Human Pluripotent Stem Cells. International Journal of Cell Biology،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-477214
Modern Language Association (MLA)
Hazeltine, Laurie B.…[et al.]. Effects of Substrate Mechanics on Contractility of Cardiomyocytes Generated from Human Pluripotent Stem Cells. International Journal of Cell Biology No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-477214
American Medical Association (AMA)
Hazeltine, Laurie B.& Simmons, Chelsey S.& Salick, Max R.& Lian, Xiaojun& Badur, Mehmet G.& Han, Wenqing…[et al.]. Effects of Substrate Mechanics on Contractility of Cardiomyocytes Generated from Human Pluripotent Stem Cells. International Journal of Cell Biology. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-477214
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477214