Molecular Cloning, Sequencing, and Characterization of a Putative Acetyl-CoA-C-acetyltransferase cDNA from a Highly Fragrant Orchid Hybrid Vanda Mimi Palmer
Joint Authors
Ong Abdullah, Janna
Namasivayam, Parameswari
Teh, Seow-Ling
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Vanda Mimi Palmer, a hybrid of Vanda Tan Chay Yan and Vanda tessellata (Roxb.) Hk.f.
ex G.
Don, is cultivated as a potted ornamental plant mainly for its fragrance rather than its look.
Plant acetyl-CoA-C-acetyltransferase (ACA) is involved in the condensation of two acetyl-CoAs to form acetoacetyl-CoA, which condenses with another acetyl-CoA to yield a crucial molecule, 3-hydroxyl-3-methylglutaryl-CoA, at the initial step of the mevalonate (MVA) pathway.
An ACA gene from vandaceous orchid has never been reported.
We describe the isolation and molecular characterization of an ACA-like gene from V.
Mimi Palmer (designated as VMPACA) to facilitate a better understanding of the terpenoid biosynthesis pathway in orchids.
The deduced VMPACA encodes a 376-amino-acid protein with a molecular weight of 39 kDa, which comprises an open reading frame of 1128 bp.
It is flanked by 87 bp of 5′-untranslated region and 174 bp of 3′-untranslated region including a poly-A tail.
Its protein sequence is 81% identical to other plant ACAs and contains a thiolase active site.
The fluctuation expression pattern of VMPACA transcript by real-time RT-PCR showed that it is developmentally and temporally regulated with predominant expression in outer and lateral inner tepals compared to vegetative tissues.
American Psychological Association (APA)
Teh, Seow-Ling& Ong Abdullah, Janna& Namasivayam, Parameswari. 2012. Molecular Cloning, Sequencing, and Characterization of a Putative Acetyl-CoA-C-acetyltransferase cDNA from a Highly Fragrant Orchid Hybrid Vanda Mimi Palmer. Sequencing،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-477273
Modern Language Association (MLA)
Teh, Seow-Ling…[et al.]. Molecular Cloning, Sequencing, and Characterization of a Putative Acetyl-CoA-C-acetyltransferase cDNA from a Highly Fragrant Orchid Hybrid Vanda Mimi Palmer. Sequencing No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-477273
American Medical Association (AMA)
Teh, Seow-Ling& Ong Abdullah, Janna& Namasivayam, Parameswari. Molecular Cloning, Sequencing, and Characterization of a Putative Acetyl-CoA-C-acetyltransferase cDNA from a Highly Fragrant Orchid Hybrid Vanda Mimi Palmer. Sequencing. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-477273
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477273