A New Approach of In Vivo Musculoskeletal Tissue Engineering Using the Epigastric Artery as Central Core Vessel of a 3-Dimensional Construct

Abstract EN

The creation of musculoskeletal tissue represents an alternative for the replacement of soft tissue in reconstructive surgery.

However, most of the approaches of creating artificial tissue have their limitations in the size as the maximally obtainable dimension of bioartificial tissue (BAT) is limited due to the lack of supporting vessels within the 3-dimensional construct.

The seeded myoblasts require high amounts of perfusion, oxygen, and nutrients to survive.

To achieve this, we developed a 3-dimensional scaffold which features the epigastric artery as macroscopic core vessel inside the BAT in a rat model (perfused group, n=4) and a control group (n=3) without the epigastric vessels and, therefore, without perfusion.

The in vivo monitoring of the transplanted myoblasts was assessed by bioluminescence imaging and showed both the viability of the epigastric artery within the 3-dimensional construct and again that cell survival in vivo is highly depending on the blood supply with the beginning of capillarization within the BAT seven days after transplantation in the perfused group.

However, further studies focussing on the matrix improvement will be necessary to create a transplantable BAT with the epigastric artery as anastomosable vessel.