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Isolation, Screening, and Identification of Cellulolytic Bacteria from Natural Reserves in the Subtropical Region of China and Optimization of Cellulase Production by Paenibacillus terrae ME27-1
Joint Authors
Feng, Jia-Xun
Liang, Yan-Ling
Zhang, Zheng
Wu, Yuan
Wu, Min
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-19
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
From different natural reserves in the subtropical region of China, a total of 245 aerobic bacterial strains were isolated on agar plates containing sugarcane bagasse pulp as the sole carbon source.
Of the 245 strains, 22 showed hydrolyzing zones on agar plates containing carboxymethyl cellulose after Congo-red staining.
Molecular identification showed that the 22 strains belonged to 10 different genera, with the Burkholderia genus exhibiting the highest strain diversity and accounting for 36.36% of all the 22 strains.
Three isolates among the 22 strains showed higher carboxymethyl cellulase (CMCase) activity, and isolate ME27-1 exhibited the highest CMCase activity in liquid culture.
The strain ME27-1 was identified as Paenibacillus terrae on the basis of 16S rRNA gene sequence analysis as well as physiological and biochemical properties.
The optimum pH and temperature for CMCase activity produced by the strain ME27-1 were 5.5 and 50°C, respectively, and the enzyme was stable at a wide pH range of 5.0–9.5.
A 12-fold improvement in the CMCase activity (2.08 U/mL) of ME27-1 was obtained under optimal conditions for CMCase production.
Thus, this study provided further information about the diversity of cellulose-degrading bacteria in the subtropical region of China and found P.
terrae ME27-1 to be highly cellulolytic.
American Psychological Association (APA)
Liang, Yan-Ling& Zhang, Zheng& Wu, Min& Wu, Yuan& Feng, Jia-Xun. 2014. Isolation, Screening, and Identification of Cellulolytic Bacteria from Natural Reserves in the Subtropical Region of China and Optimization of Cellulase Production by Paenibacillus terrae ME27-1. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-477506
Modern Language Association (MLA)
Liang, Yan-Ling…[et al.]. Isolation, Screening, and Identification of Cellulolytic Bacteria from Natural Reserves in the Subtropical Region of China and Optimization of Cellulase Production by Paenibacillus terrae ME27-1. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-477506
American Medical Association (AMA)
Liang, Yan-Ling& Zhang, Zheng& Wu, Min& Wu, Yuan& Feng, Jia-Xun. Isolation, Screening, and Identification of Cellulolytic Bacteria from Natural Reserves in the Subtropical Region of China and Optimization of Cellulase Production by Paenibacillus terrae ME27-1. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-477506
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477506