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Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium
Joint Authors
Saxena, M. K.
Tamuly, Shantanu
Saxena, Anjani
Jha, Richa
Kumar, Anil
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Salmonella Typhimurium is an important pathogen having a broad host range.
In human population it causes mostly gastroenteritis but there are reports in which it was found to be responsible to cause several lethal diseases like endocarditis and meningitis.
Poultry products are the major sources of this organism in India as these are consumed at various stages of cooking.
The available vaccines have their own limitations such as short-term immunity.
Outer membrane proteins have shown some promising potential, so in the present study Omp C of Salmonella Typhimurium was cloned and sequenced to explore the possibility of development of r-DNA vaccine against Salmonella Typhimurium for poultry.
The sequence of Omp C was studied for antigenic indexing, epitope mapping, and MHC mapping using various bioinformatic tools.
The ORF analysis revealed a complete coding region of approximately 1000 bp.
Five major and 13 minor B-cell epitopes were identified having an antigenic index of 1.7.
The sequences also showed major histocompatibility complex (MHC) class I and class II binding region indicating a potential of eliciting cell-mediated immune response.
The findings indicate that Omp C may be proven as promising candidate for development of r-DNA vaccine against Salmonella Typhimurium.
American Psychological Association (APA)
Jha, Richa& Kumar, Anil& Saxena, Anjani& Tamuly, Shantanu& Saxena, M. K.. 2012. Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium. ISRN Veterinary Science،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-477548
Modern Language Association (MLA)
Jha, Richa…[et al.]. Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium. ISRN Veterinary Science No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-477548
American Medical Association (AMA)
Jha, Richa& Kumar, Anil& Saxena, Anjani& Tamuly, Shantanu& Saxena, M. K.. Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium. ISRN Veterinary Science. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-477548
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477548