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An Approach to Visualize the Deformation of the Intermediate Filament Cytoskeleton in Response to Locally Applied Forces
Joint Authors
Wang, Jiashan
Pelling, Andrew E.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The intermediate filament (IF) cytoskeleton plays an important role in integrating biomechanical pathways associated with the actin and microtubule cytoskeleton.
Vimentin is a type III IF protein commonly found in fibroblast cells and plays a role in transmitting forces through the cytoskeleton.
Employing simultaneous laser scanning confocal and atomic force microscopy (AFM), we developed a methodology to quantify the deformation of the GFP-vimentin-labeled IF cytoskeleton as a function of time in response to force application by the AFM.
Over short times (seconds), IFs deformed rapidly and transmitted force throughout the entire cell in a highly complex and anisotropic fashion.
After several minutes, mechanically induced displacements of IFs resemble basal movements.
In well-adhered cells the deformation of IFs is highly anisotropic as they tend to deform away from the longitudinal axis of the cell.
This study demonstrates that simultaneous AFM and LSCM can be employed to track the deformation and dissipation of force through the IF cytoskeleton.
American Psychological Association (APA)
Wang, Jiashan& Pelling, Andrew E.. 2011. An Approach to Visualize the Deformation of the Intermediate Filament Cytoskeleton in Response to Locally Applied Forces. ISRN Cell Biology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-477614
Modern Language Association (MLA)
Wang, Jiashan& Pelling, Andrew E.. An Approach to Visualize the Deformation of the Intermediate Filament Cytoskeleton in Response to Locally Applied Forces. ISRN Cell Biology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-477614
American Medical Association (AMA)
Wang, Jiashan& Pelling, Andrew E.. An Approach to Visualize the Deformation of the Intermediate Filament Cytoskeleton in Response to Locally Applied Forces. ISRN Cell Biology. 2011. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-477614
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477614