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Influence of a Hairpin Loop on the Thermodynamic Stability of a DNA Oligomer
Joint Authors
Vesnaver, Gorazd
Lah, Jurij
Seručnik, Mojca
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
DSC was used to evaluate the mechanism of the thermally induced unfolding of the single-stranded hairpin HP = 5′-CGGAATTCCGTCTCCGGAATTCCG-3′ and its core duplex D (5′-CGGAATTCCG-3′)2.
The DSC melting experiments performed at several salt concentrations were successfully described for HP and D in terms of a three-state transition model HP↔I (intermediate state) ↔ S (unfolded single-stranded state) and two state transition model D↔2S, respectively.
Comparison of the model-based thermodynamic parameters obtained for each HP and D transition shows that in unfolding of HP only the HP↔I transition is affected by the TCTC loop.
This observation suggests that in the intermediate state its TCTC loop part exhibits significantly more flexible structure than in the folded state while its duplex part remains pretty much unchanged.
American Psychological Association (APA)
Lah, Jurij& Seručnik, Mojca& Vesnaver, Gorazd. 2011. Influence of a Hairpin Loop on the Thermodynamic Stability of a DNA Oligomer. Journal of Nucleic Acids،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-477652
Modern Language Association (MLA)
Lah, Jurij…[et al.]. Influence of a Hairpin Loop on the Thermodynamic Stability of a DNA Oligomer. Journal of Nucleic Acids No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-477652
American Medical Association (AMA)
Lah, Jurij& Seručnik, Mojca& Vesnaver, Gorazd. Influence of a Hairpin Loop on the Thermodynamic Stability of a DNA Oligomer. Journal of Nucleic Acids. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-477652
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477652