Internally Controlled, Generic Real-Time PCR for Quantification and Multiplex Real-Time PCR with Serotype-Specific Probes for Serotyping of Dengue Virus Infections
Joint Authors
Menting, Sandra
Phuong, Hoang L.
de Vries, Peter J.
Thai, Khoa T. D.
Binh, Tran Q.
Beld, Marcel
Klatser, Paul
Nga, Tran T. T.
Wolthers, Katja C.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Dengue has become a global public health problem and a sensitive diagnostic test for early phase detection can be life saving.
An internally controlled, generic real-time PCR was developed and validated by testing serial dilutions of a DENV positive control RNA in the presence of a fixed amount of IC with results showing a good linearity (R2=0.9967) and a LOD of at least 1.95×104 copies/mL.
Application of the generic PCR on 136 patient samples revealed a sensitivity of 95.8% and specificity of 100%.
A newly developed multiplex real-time PCR with serotype-specific probes allowed the serotyping of DENV for 80 out of 92 (87%) generic real-time PCR positive patients.
Combined these real-time PCRs offer a convenient diagnostic tool for the sensitive and specific quantification of DENV in clinical specimens with the possibility for serotyping.
American Psychological Association (APA)
Menting, Sandra& Thai, Khoa T. D.& Nga, Tran T. T.& Phuong, Hoang L.& Klatser, Paul& Wolthers, Katja C.…[et al.]. 2011. Internally Controlled, Generic Real-Time PCR for Quantification and Multiplex Real-Time PCR with Serotype-Specific Probes for Serotyping of Dengue Virus Infections. Advances in Virology،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-477723
Modern Language Association (MLA)
Menting, Sandra…[et al.]. Internally Controlled, Generic Real-Time PCR for Quantification and Multiplex Real-Time PCR with Serotype-Specific Probes for Serotyping of Dengue Virus Infections. Advances in Virology No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-477723
American Medical Association (AMA)
Menting, Sandra& Thai, Khoa T. D.& Nga, Tran T. T.& Phuong, Hoang L.& Klatser, Paul& Wolthers, Katja C.…[et al.]. Internally Controlled, Generic Real-Time PCR for Quantification and Multiplex Real-Time PCR with Serotype-Specific Probes for Serotyping of Dengue Virus Infections. Advances in Virology. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-477723
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477723
