Application of iTRAQ Reagents to Relatively Quantify the Reversible Redox State of Cysteine Residues
Joint Authors
Vázquez, Jesús
Sheehan, David
Martínez-Acedo, Pablo
Padilla, C. Alicia
Bárcena, José Antonio
McDonagh, Brian
Source
International Journal of Proteomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Cysteines are one of the most rarely used amino acids, but when conserved in proteins they often play critical roles in structure, function, or regulation.
Reversible cysteine modifications allow for potential redox regulation of proteins.
Traditional measurement of the relative absolute quantity of a protein between two samples is not always necessarily proportional to the activity of the protein.
We propose application of iTRAQ reagents in combination with a previous thiol selection method to relatively quantify the redox state of cysteines both within and between samples in a single analysis.
Our method allows for the identification of the proteins, identification of redox-sensitive cysteines within proteins, and quantification of the redox status of individual cysteine-containing peptides.
As a proof of principle, we applied this technique to yeast alcohol dehydrogenase-1 exposed in vitro to H2O2 and also in vivo to the complex proteome of the Gram-negative bacterium Bacillus subtilis.
American Psychological Association (APA)
McDonagh, Brian& Martínez-Acedo, Pablo& Vázquez, Jesús& Padilla, C. Alicia& Sheehan, David& Bárcena, José Antonio. 2012. Application of iTRAQ Reagents to Relatively Quantify the Reversible Redox State of Cysteine Residues. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-477747
Modern Language Association (MLA)
McDonagh, Brian…[et al.]. Application of iTRAQ Reagents to Relatively Quantify the Reversible Redox State of Cysteine Residues. International Journal of Proteomics No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-477747
American Medical Association (AMA)
McDonagh, Brian& Martínez-Acedo, Pablo& Vázquez, Jesús& Padilla, C. Alicia& Sheehan, David& Bárcena, José Antonio. Application of iTRAQ Reagents to Relatively Quantify the Reversible Redox State of Cysteine Residues. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-477747
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477747