Functional Proteomic Profiling of Phosphodiesterases Using SeraFILE Separations Platform
Joint Authors
Kuruc, Matthew P.
Gujarathi, Ketan M.
Roy, Swapan
Oka, Amita R.
Source
International Journal of Proteomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Functional proteomic profiling can help identify targets for disease diagnosis and therapy.
Available methods are limited by the inability to profile many functional properties measured by enzymes kinetics.
The functional proteomic profiling approach proposed here seeks to overcome such limitations.
It begins with surface-based proteome separations of tissue/cell-line extracts, using SeraFILE, a proprietary protein separations platform.
Enzyme kinetic properties of resulting subproteomes are then characterized, and the data integrated into proteomic profiles.
As a model, SeraFILE-derived subproteomes of cyclic nucleotide-hydrolyzing phosphodiesterases (PDEs) from bovine brain homogenate (BBH) and rat brain homogenate (RBH) were characterized for cAMP hydrolysis activity in the presence (challenge condition) and absence of cGMP.
Functional profiles of RBH and BBH were compiled from the enzyme activity response to the challenge condition in each of the respective subproteomes.
Intersample analysis showed that comparable profiles differed in only a few data points, and that distinctive subproteomes can be generated from comparable tissue samples from different animals.
These results demonstrate that the proposed methods provide a means to simplify intersample differences, and to localize proteins attributable to sample-specific responses.
It can be potentially applied for disease and nondisease sample comparison in biomarker discovery and drug discovery profiling.
American Psychological Association (APA)
Oka, Amita R.& Kuruc, Matthew P.& Gujarathi, Ketan M.& Roy, Swapan. 2012. Functional Proteomic Profiling of Phosphodiesterases Using SeraFILE Separations Platform. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-477788
Modern Language Association (MLA)
Oka, Amita R.…[et al.]. Functional Proteomic Profiling of Phosphodiesterases Using SeraFILE Separations Platform. International Journal of Proteomics No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-477788
American Medical Association (AMA)
Oka, Amita R.& Kuruc, Matthew P.& Gujarathi, Ketan M.& Roy, Swapan. Functional Proteomic Profiling of Phosphodiesterases Using SeraFILE Separations Platform. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-477788
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-477788