In Vitro Antifungal Evaluation of Seven Different Disinfectants on Acrylic Resins

Abstract EN

Objective.

The aim of this study was to evaluate alternative methods for the disinfection of denture-based materials.

Material and Methods.

Two different denture-based materials were included in the study.

Before microbial test, the surface roughness of the acrylic resins was evaluated.

Then, the specimens were divided into 8 experimental groups (n=10), according to microorganism considered and disinfection methods used.

The specimens were contaminated in vitro by standardized suspensions of Candida albicans ATCC#90028 and Candida albicans oral isolate.

The following test agents were tested: sodium hypochlorite (NaOCl 1%), microwave (MW) energy, ultraviolet (UV) light, mouthwash containing propolis (MCP), Corega Tabs, 50% and 100% white vinegar.

After the disinfection procedure, the number of remaining microbial cells was evaluated in CFU/mL.

Kruskal-Wallis, ANOVA, and Dunn’s test were used for multiple comparisons.

Mann Whitney U test was used to compare the surface roughness.

Results.

Statistically significant difference (P<0.05) was found between autopolymerised and heat-cured acrylic resins.

The autopolymerised acrylic resin surfaces were rougher than surfaces of heat-cured acrylic resin.

The most effective disinfection method was 100% white vinegar for tested microorganisms and both acrylic resins.

Conclusion.

This study showed that white vinegar 100% was the most effective method for tested microorganisms.

This agent is cost-effective and easy to access and thus may be appropriate for household use.