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Altered Proteolysis in Fibroblasts of Alzheimer Patients with Predictive Implications for Subjects at Risk of Disease
Joint Authors
Sanchez, Luis
Pani, Alessandra
Mocali, Alessandra
Boddi, Vieri
Della Malva, Nunzia
Abete, Claudia
Mitidieri Costanza, Vito Antonio
Bavazzano, Antonio
Dessì, Sandra
Paoletti, Francesco
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
There is great interest in developing reliable biomarkers to support antemortem diagnosis of late-onset Alzheimer’s disease (AD).
Early prediction and diagnosis of AD might be improved by the detection of a proteolytic dysfunction in extracts from cultured AD fibroblasts, producing altered isoelectrophoretic forms of the enzyme transketolase (TK-alkaline bands).
The TK profile and apolipoprotein E (APOE) genotype were examined in fibroblasts from 36 clinically diagnosed probable late-onset sporadic AD patients and 38 of their asymptomatic relatives, 29 elderly healthy individuals, 12 neurological non-AD patients, and 5 early-onset AD patients.
TK alterations occurred in (i) several probable AD patients regardless of age-of-onset and severity of disease; (ii) all early-onset AD patients and APOE ε4/4 carriers; and (iii) nearly half of asymptomatic AD relatives.
Normal subjects and non-AD patients were all negative.
Notably, culture conditions promoting TK alterations were also effective in increasing active BACE1 levels.
Overall, the TK assay might represent a low-cost laboratory tool useful for supporting AD differential diagnosis and identifying asymptomatic subjects who are at greater risk of AD and who should enter a follow-up study.
Moreover, the cultured fibroblasts were confirmed as a useful in vitro model for further studies on the pathogenetic process of AD.
American Psychological Association (APA)
Mocali, Alessandra& Della Malva, Nunzia& Abete, Claudia& Mitidieri Costanza, Vito Antonio& Bavazzano, Antonio& Boddi, Vieri…[et al.]. 2014. Altered Proteolysis in Fibroblasts of Alzheimer Patients with Predictive Implications for Subjects at Risk of Disease. ISRN Algebra،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-478227
Modern Language Association (MLA)
Mocali, Alessandra…[et al.]. Altered Proteolysis in Fibroblasts of Alzheimer Patients with Predictive Implications for Subjects at Risk of Disease. ISRN Algebra No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-478227
American Medical Association (AMA)
Mocali, Alessandra& Della Malva, Nunzia& Abete, Claudia& Mitidieri Costanza, Vito Antonio& Bavazzano, Antonio& Boddi, Vieri…[et al.]. Altered Proteolysis in Fibroblasts of Alzheimer Patients with Predictive Implications for Subjects at Risk of Disease. ISRN Algebra. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-478227
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478227