Comparative Analysis and EST Mining Reveals High Degree of Conservation among Five Brassicaceae Species
Joint Authors
Jhang, Tripta
Singh, Nagender Kumar
Bhati, Jyotika
Sonah, Humira
Sharma, Tilak Raj
Source
Comparative and Functional Genomics
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Brassicaceae is an important family of the plant kingdom which includes several plants of major economic importance.
The Brassica spp.
and Arabidopsis share much-conserved colinearity between their genomes which can be exploited for the genomic research in Brassicaceae crops.
In this study, 131,286 ESTs of five Brassicaceae species were assembled into unigene contigs and compared with Arabidopsis gene indices.
Almost all the unigenes of Brassicaceae species showed high similarities with Arabidopsis genes except those of B.
napus, where 90% of unigenes were found similar.
A total of 9,699 SSRs were identified in the unigenes.
PCR primers were designed based on this information and amplified across species for validation.
Functional annotation of unigenes showed that the majority of the genes are present in metabolism and energy functional classes.
It is expected that comparative genome analysis between Arabidopsis and related crop species will expedite research in the more complex Brassica genomes.
This would be helpful for genomics as well as evolutionary studies, and DNA markers developed can be used for mapping, tagging, and cloning of important genes in Brassicaceae.
American Psychological Association (APA)
Bhati, Jyotika& Sonah, Humira& Jhang, Tripta& Singh, Nagender Kumar& Sharma, Tilak Raj. 2010. Comparative Analysis and EST Mining Reveals High Degree of Conservation among Five Brassicaceae Species. Comparative and Functional Genomics،Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-478237
Modern Language Association (MLA)
Bhati, Jyotika…[et al.]. Comparative Analysis and EST Mining Reveals High Degree of Conservation among Five Brassicaceae Species. Comparative and Functional Genomics No. 2010 (2010), pp.1-13.
https://search.emarefa.net/detail/BIM-478237
American Medical Association (AMA)
Bhati, Jyotika& Sonah, Humira& Jhang, Tripta& Singh, Nagender Kumar& Sharma, Tilak Raj. Comparative Analysis and EST Mining Reveals High Degree of Conservation among Five Brassicaceae Species. Comparative and Functional Genomics. 2010. Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-478237
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478237