UniPrimer : A Web-Based Primer Design Tool for Comparative Analyses of Primate Genomes
Joint Authors
Han, Kyudong
Lee, Jungnam
Oh, Sejong
Hong, Seung Bok
Lee, Jimin
Batnyam, Nomin
Source
Comparative and Functional Genomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Whole genome sequences of various primates have been released due to advanced DNA-sequencing technology.
A combination of computational data mining and the polymerase chain reaction (PCR) assay to validate the data is an excellent method for conducting comparative genomics.
Thus, designing primers for PCR is an essential procedure for a comparative analysis of primate genomes.
Here, we developed and introduced UniPrimer for use in those studies.
UniPrimer is a web-based tool that designs PCR- and DNA-sequencing primers.
It compares the sequences from six different primates (human, chimpanzee, gorilla, orangutan, gibbon, and rhesus macaque) and designs primers on the conserved region across species.
UniPrimer is linked to RepeatMasker, Primer3Plus, and OligoCalc softwares to produce primers with high accuracy and UCSC In-Silico PCR to confirm whether the designed primers work.
To test the performance of UniPrimer, we designed primers on sample sequences using UniPrimer and manually designed primers for the same sequences.
The comparison of the two processes showed that UniPrimer was more effective than manual work in terms of saving time and reducing errors.
American Psychological Association (APA)
Batnyam, Nomin& Lee, Jimin& Lee, Jungnam& Hong, Seung Bok& Oh, Sejong& Han, Kyudong. 2012. UniPrimer : A Web-Based Primer Design Tool for Comparative Analyses of Primate Genomes. Comparative and Functional Genomics،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-478282
Modern Language Association (MLA)
Batnyam, Nomin…[et al.]. UniPrimer : A Web-Based Primer Design Tool for Comparative Analyses of Primate Genomes. Comparative and Functional Genomics No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-478282
American Medical Association (AMA)
Batnyam, Nomin& Lee, Jimin& Lee, Jungnam& Hong, Seung Bok& Oh, Sejong& Han, Kyudong. UniPrimer : A Web-Based Primer Design Tool for Comparative Analyses of Primate Genomes. Comparative and Functional Genomics. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-478282
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478282