Incorporation of 3-Aminobenzanthrone into 2′-Deoxyoligonucleotides and Its Impact on Duplex Stability
Joint Authors
Zaliznyak, Tanya
Lukin, Mark
Johnson, Francis
de los Santos, Carlos R.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
3-Nitrobenzanthrone (3NBA), an environmental pollutant and potent mutagen, causes DNA damage via the reaction of its metabolically activated form with the exocyclic amino groups of purines and the C-8 position of guanine.
The present work describes a synthetic approach to the preparation of oligomeric 2′-deoxyribonucleotides containing a 2-(2′-deoxyguanosin-N2-yl)-3-aminobenzanthrone moiety, one of the major DNA adducts found in tissues of living organisms exposed to 3NBA.
The NMR spectra indicate that the damaged oligodeoxyribonucleotide is capable of forming a regular double helical structure with the polyaromatic moiety assuming a single conformation at room temperature; the spectra suggest that the 3ABA moiety resides in the duplex minor groove pointing toward the 5′-end of the modified strand.
Thermodynamic studies show that the dG(N2)-3ABA lesion has a stabilizing effect on the damaged duplex, a fact that correlates well with the long persistence of this damage in living organisms.
American Psychological Association (APA)
Lukin, Mark& Zaliznyak, Tanya& Johnson, Francis& de los Santos, Carlos R.. 2011. Incorporation of 3-Aminobenzanthrone into 2′-Deoxyoligonucleotides and Its Impact on Duplex Stability. Journal of Nucleic Acids،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-478315
Modern Language Association (MLA)
Lukin, Mark…[et al.]. Incorporation of 3-Aminobenzanthrone into 2′-Deoxyoligonucleotides and Its Impact on Duplex Stability. Journal of Nucleic Acids No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-478315
American Medical Association (AMA)
Lukin, Mark& Zaliznyak, Tanya& Johnson, Francis& de los Santos, Carlos R.. Incorporation of 3-Aminobenzanthrone into 2′-Deoxyoligonucleotides and Its Impact on Duplex Stability. Journal of Nucleic Acids. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-478315
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478315