Aldo-Keto Reductases in the Eye
Joint Authors
Varma, Ross Arjun
Reddy, Geereddy Bhanuprakash
Huang, Shun Ping
Petrash, J. Mark
Harter, Theresa
Palla, Suryanarayana
Ruzycki, Philip
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-13
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Aldose reductase (AKR1B1) is an NADPH-dependent aldo-keto reductase best known as the rate-limiting enzyme of the polyol pathway.
Accelerated glucose metabolism through this pathway has been implicated in diabetic cataract and retinopathy.
Some human tissues contain AKR1B1 as well as AKR1B10, a closely related member of the aldo-keto reductase gene superfamily.
This opens the possibility that AKR1B10 may also contribute to diabetic complications.
The goal of the current study was to characterize the expression profiles of AKR1B1 and AKR1B10 in the human eye.
Using quantitative reverse transcriptase-PCR and immunohistochemical staining, we observed expression of both AKR genes in cornea, iris, ciliary body, lens, and retina.
Expression of AKR1B1 was the highest in lens and retina, whereas AKR1B10 was the highest in cornea.
Lenses from transgenic mice designed for overexpression of AKR1B10 were not significantly different from nontransgenic controls, although a significant number developed a focal defect in the anterior lens epithelium following 6 months of experimentally induced diabetes.
However, lenses from AKR1B10 mice remained largely transparent following longterm diabetes.
These results indicate that AKR1B1 and AKR1B10 may have different functional properties in the lens and suggest that AKR1B10 does not contribute to the pathogenesis of diabetic cataract in humans.
American Psychological Association (APA)
Huang, Shun Ping& Palla, Suryanarayana& Ruzycki, Philip& Varma, Ross Arjun& Harter, Theresa& Reddy, Geereddy Bhanuprakash…[et al.]. 2010. Aldo-Keto Reductases in the Eye. Journal of Ophthalmology،Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-478324
Modern Language Association (MLA)
Huang, Shun Ping…[et al.]. Aldo-Keto Reductases in the Eye. Journal of Ophthalmology No. 2010 (2010), pp.1-6.
https://search.emarefa.net/detail/BIM-478324
American Medical Association (AMA)
Huang, Shun Ping& Palla, Suryanarayana& Ruzycki, Philip& Varma, Ross Arjun& Harter, Theresa& Reddy, Geereddy Bhanuprakash…[et al.]. Aldo-Keto Reductases in the Eye. Journal of Ophthalmology. 2010. Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-478324
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478324