Genotypic Characterization of Yersinia enterocolitica Biotype 4O : 3 Isolates from Pigs and Slaughterhouses Using SE-AFLP, ERIC-PCR, and PFGE
Joint Authors
Spindola, Maria Garcia
dos Reis, Cristhiane Moura Falavina
Gobbi, Debora Dirani Sena de
Porfida Ferreira, Thais Sebastiana
Paixão, Renata
Hofer, Ernesto
Matté, Maria Helena
Moreno, Luisa Zanolli
Moreno, Andrea Micke
Raimundo, Daniele Cristine
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Yersinia enterocolitica is a foodborne pathogen that causes illness in humans and animals.
The biotype 4/O:3 has been commonly associated with yersiniosis and is characterized by the presence of chromosomal and extra-chromosomal virulence genes.
Molecular typing methods have been successfully used to characterize Y.
enterocolitica genetic heterogeneity and to study the epidemiology of the bacteria from different origins.
In this study, 320 Y.
enterocolitica biotype 4/O:3 isolates originating in pigs and slaughterhouses were characterized according to the virulence profile, and 61 isolates were typified through SE-AFLP, ERIC-PCR, and PFGE techniques.
The majority of the isolates originated from pigs, and the predominant virulence profile was ail+ virF+ rfbC+ ystA+, representing 83.4% of the tested isolates.
All of the Y.
enterocolitica 4/O:3 isolates were positive for at least ystA gene.
The SE-AFLP and ERIC-PCR patterns were highly homogeneous.
The SE-AFLP was more discriminative than the ERIC-PCR and tended to cluster isolates according to the slaughterhouse.
Despite the limited genetic diversity of Y.
enterocolitica 4/O:3, PFGE was shown to be the most discriminative technique considering one band of difference.
Fattening pigs proved to be an important reservoir of Y.
enterocolitica biotype 4/O:3 carrying virulence genes.
American Psychological Association (APA)
Paixão, Renata& Moreno, Luisa Zanolli& Gobbi, Debora Dirani Sena de& Raimundo, Daniele Cristine& Porfida Ferreira, Thais Sebastiana& Spindola, Maria Garcia…[et al.]. 2013. Genotypic Characterization of Yersinia enterocolitica Biotype 4O : 3 Isolates from Pigs and Slaughterhouses Using SE-AFLP, ERIC-PCR, and PFGE. Journal of Pathogens،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-478354
Modern Language Association (MLA)
Paixão, Renata…[et al.]. Genotypic Characterization of Yersinia enterocolitica Biotype 4O : 3 Isolates from Pigs and Slaughterhouses Using SE-AFLP, ERIC-PCR, and PFGE. Journal of Pathogens No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-478354
American Medical Association (AMA)
Paixão, Renata& Moreno, Luisa Zanolli& Gobbi, Debora Dirani Sena de& Raimundo, Daniele Cristine& Porfida Ferreira, Thais Sebastiana& Spindola, Maria Garcia…[et al.]. Genotypic Characterization of Yersinia enterocolitica Biotype 4O : 3 Isolates from Pigs and Slaughterhouses Using SE-AFLP, ERIC-PCR, and PFGE. Journal of Pathogens. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-478354
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478354