PTH Assays : Understanding What We Have and Forecasting What We Will Have
Author
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-03
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Parathyroid hormone (PTH) assays have evolved continuously for the last 50 years.
Since the first radioimmunoassay was described in 1963, several assays based on immunological identification have been published (first generation assays).
The routine assays used nowadays are immunometric “sandwich-type”.
They are based on two different monoclonal antibodies, one amino-terminal and the other carboxyl terminal specific.
These second generation assays are widely available and adapted to most of the automation platforms.
The specificity of the amino terminal antibody defines if the immunometric assay measures only the bioactive PTH circulating form (including the first amino terminal amino acids) or the “intact” PTH, which includes, besides bioactive PTH, other “long” carboxyl-terminal forms, for example, 7–84-PTH.
Assays for “intact” PTH are the most commonly available and the potential advantage of the bioactive PTH assays is still debatable.
Next generation of assays will be based on different principles, mainly mass spectrometry in samples submitted to a prior purification and fragmentation steps.
These assays will provide information about the whole spectra of PTH peptides in circulation, with a significant increase of the information regarding this biologically important peptide hormone.
American Psychological Association (APA)
Vieira, Jose Gilberto H.. 2012. PTH Assays : Understanding What We Have and Forecasting What We Will Have. Journal of Osteoporosis،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-478421
Modern Language Association (MLA)
Vieira, Jose Gilberto H.. PTH Assays : Understanding What We Have and Forecasting What We Will Have. Journal of Osteoporosis No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-478421
American Medical Association (AMA)
Vieira, Jose Gilberto H.. PTH Assays : Understanding What We Have and Forecasting What We Will Have. Journal of Osteoporosis. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-478421
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-478421